ABUNDANT EXPRESSION OF NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) GENE IN GOLDFISH SCALE WITH LATERAL LINE
スポンサーリンク
概要
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To explore the possible utilization of goldfish scale in monitoring environmental toxicants as well as that for calcified tissue research, characteristics of cells in the scale were examined by identifying genes differentially expressed in those cells. Subtraction cloning was subjected to RNAs between scale-gill (SG), gill-scale (GS) and scale with lateral line-scale without lateral line (LS). Total numbers of 4, 6, and 9 clones were isolated respectively from SG, GS and LS pools. Blast search of their sequence showed only low homology to other fish sequences so that 5' rapid amplification of cDNA (5'RACE) was applied to the obtained 5' terminal side of each sequence. Among 19 clones, LS3 alone showed the high homology to zebrafish nucleosome assembly protein 1 (NAP1) and the rest of 18 was not yet identified. In situ hybridization confirmed that faint expression of NAP1 mRNA was observed in cells along the ridge of the scale without lateral line. On the other hand, in small cells not along the ridge of the scale with lateral line, very intense hybridization was found as expected. They were abundant at the surrounding area of the lateral line tube. NAP1 plays an enhancing role on gene expression by promoting nucleosome assembly. Thus, cell viability in the scale with lateral line seemed to be higher than that in the scale without lateral line. The scale can be a good model to monitor the effects of environmental pollutants because simultaneous observation against cells with different cell viability is available through comparison between cells in the scale with or without lateral line.
- 日本トキシコロジー学会の論文
- 2007-10-15
著者
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SAIJOH Kiyofumi
Department of Hygiene, Kanazawa University School of Medicine and Graduate School of Medical Science
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Tanii Hideji
Department of Hygiene, Kanazawa University Graduate School of Medical Science
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KATSUYAMA Hironobu
Department of Public Health, Kawasaki Medical College
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Katsuyama Hironobu
Department Of Public Health Kawasaki Medical University
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Saijoh Kiyofumi
Department Of Hygiene Kanazawa University School Of Medicine And Graduate School Of Medical Sciences
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Saijoh Kiyofumi
Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine
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LI LiHua
Department of Environmental and Molecular Bio-Informatics, Kanazawa University School of Medicine
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DO Son
Department of Environmental and Molecular Bio-Informatics, Kanazawa University School of Medicine
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SAITO Masayuki
Department of Environmental and Molecular Bio-Informatics, Kanazawa University School of Medicine
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Do Son
Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine
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Do Son
金沢大学 医学部環境生体分子応答学
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Tanii Hideji
Department Of Hygiene Kanazawa University Graduate School Of Medical Science
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Tanii Hideji
Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine
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Li Lihua
Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine
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Katsuyama Hironobu
Department Of Public Health Kawasaki Medical College
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Saito Masayuki
Department Of Chemistry Graduate School Of Science Osaka University
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Saito Masayuki
Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine
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SAITO Masayuki
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
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SAITO Masayuki
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University
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