Multiple Variant mRNAs with Different Length Tandem Repeats of (CAYYCC)n Produced from Bovine Selenoprotein P-like Protein Gene

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In contrast to selenoprotein Ps (SelPs) from other animal species, bovine selenoprotein P-like-protein (SelPLP) was found to contain a tandem repeat of (CAYYCC)_11. During an investigation into whether SelPLP was a bovine substitute for SelP or uniquely bovine, its mRNA was found to consist of multiple variants with different length tandem repeat, namely p(0) with (CAYYCC)_11, p(-4) lacking (CAYYCC)_4, p(-8) lacking (CAYYCC)_8, and p(-9) lacking (CAYYCC)_9. Although they were encoded on a single gene locus, neither classical GT-AG nor minor class AT-AC donator-acceptor sequences for alternative splicing were identified. A subsequent S1 protection assay using oligonucleotides, whose sequence may occur as variants, performed against bovine poly(A)^+RNA identified a total of nine variants. Judging from the sequence of these variants and the branch point mapping, the consensus sequence for recognition of the donator was CACCCC_<CAC> and of the acceptor and the branch point A nucleotide, <ACCCC>_CAT or <ACCCC>_CATCCCCAT. Furthermore, when the p(0) insert mRNA was expressed in COS-7 cells derived from an African green monkey kidney, cDNAs corresponding to p(-8) and p(-9) could be isolated. Therefore, the bovine SelPLP mRNAs consisted of multiple variants probably due to a novel splicing mechanism which was not bovine-specific but common to other mammals.
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