Detection of Differentially Expressed Genes in Esophageal Carcinoma Using Non-RI Differential Display with High Specificity

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概要

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• Differentially expressed genes in human esophageal carcinoma/normal tissue pairs were identified by means of a modified differential display technique to overcome the limitations of the conventional technique. Out of the eight cDNAs isolated, three were novel. The other five clones consisted of cDNA encoding cytokeratin 13, complement 7, KIAA1160, expression sequence tag (EST)-Hs110855 and EST-Hs13662. Quantitative reverse transcription-PCR (RT-PCR) for 10 carcinoma/normal tissue pairs confirmed down-regulation of cytokeratin 13, complement 7 and KIAA1160 mRNAs in carcinoma tissue and up-regulation of EST-Hs110855 mRNA. EST-Hs13662 mRNA also seemed to increase in carcinoma tissues but not to a statistically significant extent. In situ hybridization confirmed that cytokeratin 13 mRNAs localized in differentiating keratinocytes of normal epithelia and had disappeared in carcinomas, suggesting that this down-regulation reflects de-differentiation during carcinogenesis. The functions of complement 7 and KIAA1160 mRNAs are unclear in normal tissue but their down-regulation in carcinoma tissue may help the development of esophageal carcinoma. The expression of EST-Hs110855 mRNA reportedly observed in carcinomas of different origins suggests that this EST is a carcinogenesis-related gene. Our modified technique, which eliminates the source of false-positives, reduces the screening time, and dispense with the radioisotope, was found to be useful for isolating differentially expressed genes from clinical specimens with apparently genetically distant cellular populations and a very limited mass.

• 社団法人日本薬学会の論文
• 2001-02-01

著者

• SAIJOH Kiyofumi

  Department of Hygiene, Kanazawa University School of Medicine and Graduate School of Medical Science

• Tanii Hideji

  Department of Hygiene, Kanazawa University Graduate School of Medical Science

• OKA Rie

  Department of Internal Medicine, Hokuriku Central Hospital

• Oka Rie

  金沢大学 衛生

• Oka R

  Department Of Hygiene Kanazawa University School Of Medicine

• Oka Rie

  Department Of Hygiene Kanazawa University School Of Medicine

• Saijoh Kiyofumi

  Department Of Hygiene Kanazawa University School Of Medicine And Graduate School Of Medical Sciences

• Saijoh Kiyofumi

  Department Of Hygiene Kanazawa University School Of Medicine

• Saijoh Kiyofumi

  Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine

• Ninomiya Itaru

  Second Department of Surgery, Kanazawa University School of Medicine

• Miwa Kouichi

  Second Department of Surgery, Kanazawa University School of Medicine

• Tanii Hideji

  金沢大学 医学系研究科衛生学

• Tanii Hideji

  Department Of Hygiene Kanazawa University Graduate School Of Medical Science

• Tanii H

  Department Of Hygiene Kanazawa University School Of Medicine

• Tanii Hideji

  Department Of Environmental And Molecular Bio-informatics Kanazawa University School Of Medicine

• Miwa Kouichi

  Second Department Of Surgery Kanazawa University School Of Medicine

• Ninomiya Itaru

  Second Department Of Surgery Kanazawa University School Of Medicine

• Tanii Hisashi

  Mie Univ. Mie Jpn

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