Binding of Norovirus Virus-Like Particles (VLPs) to Human Intestinal Caco-2 Cells and the Suppressive Effect of Pasteurized Bovine Colostrum on This VLP Binding
スポンサーリンク
概要
- 論文の詳細を見る
Noroviruses (NoVs), which cannot be grown in cell culture, are a major infectious agent of gastroenteritis. An in vitro assay system was established for the evaluation of NoV binding to enterocytes using virus-like particles (VLPs) produced in a baculovirus system expressing a NoV VP1 capsid protein. After confirmation of the purity by MS analysis, VLPs were incubated with human intestinal Caco-2 cells. NoV VLPs were detected clearly by confocal laser microscopy only on a certain population of Caco-2 cells, and were semi-quantified by immunoblotting of cell lysates. Then the suppressive effect of pasteurized bovine colostrum was analyzed on the VLP binding to Caco-2 cells by immunoblotting. The colostrum reduced VLP binding in a dose-dependent manner, at about 50% suppression with 12.5 μg of the colostral proteins. Furthermore, the colostrum contained IgG antibodies reacting to VLPs, suggesting that cross-reactive antibodies in the bovine colostrums block human NoV binding to intestinal cells.
著者
-
Aoki Naohito
Department of Life Sciences, Faculty of Bioresources, Mie University
-
KATAYAMA KAZUHIKO
Department of Internal Medicine, Keio University Hoipital
-
MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
-
Uchida Kenji
Central Research Laboratory Hitachi Ltd.
-
Takeda Naokazu
Department Of Gastroenterology Shinshu University School Of Medicine
-
Aoki Naohito
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
-
NADANO Daita
Department of Legal Medicine, Fukui Medical School
-
OKAJIMA Tetsuya
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
OKA Tomoichiro
Department of Virology II, National Institute of Infectious Diseases
-
Suzuki Sayaka
Department Of Chemistry For Material Faculty Of Engineering Mie University
-
MURAKAMI Kosuke
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
YAMASHITA Kousaku
Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
関連論文
- Comparison of Hepatitis B Virus DNA, RNA, and Core Related Antigen as Predictors of Lamivudine Resistance in Patients with Chronic Hepatitis B
- Binding of Norovirus Virus-Like Particles (VLPs) to Human Intestinal Caco-2 Cells and the Suppressive Effect of Pasteurized Bovine Colostrum on This VLP Binding
- Epidemiology of hepatocellular carcinoma in Japan
- Receptor-Type Protein Tyrosine Phosphatase ε (PTPεM) is a Negative Regulator of Insulin Signaling in Primary Hepatocytes and Liver
- Serum Fragmented Cytokeratin 18 Levels Reflect the Histologic Activity Score of Nonalcoholic Fatty Liver Disease More Accurately Than Serum Alanine Aminotransferase Levels
- A Case of Granulocyte-Colony Stimulating Factor-Producing Hepatocellular Carcinoma Confirmed by Immunohistochemistry
- SUPRAVALVULAR AORTIC STENOSIS ASSSOCIATED WITHAORTIC ANEURYSM
- Clinical and Experimental Studies on Pulmonary and Coronary Circulation : PROCEEDINGS OF THE 25TH ANNUAL MEETING JAPANESE CIRCULATION SOCIETY (Part II)
- A2BP1 as a novel susceptible gene for primary biliary cirrhosis in Japanese patients
- Purification and Properties of an Allergenic Protein in Rice Grain(Food & Nutrition)
- Impact of ω-5 Gliadin on Wheat-Dependent Exercise-Induced Anaphylaxis in Mice
- Saturation Mutagenesis reveals that GLU54 of Norovirus 3C-like Protease is not Essential for the Proteolytic Activity
- Reliability of 780-nm High-Power Laser Diodes with Thin Quantum Well Active Layer
- Deoxyribonuclease I from Rat Urine: Affinity Purification, Characterization, and Immunochemical Studies
- Correlation of Alpha-1-Antitrypsin (PI) Polymorphism between Blood and Semen
- Plantaricin-149,a Bacteriocin Produced by Lactobacillus plantarum NRIC 149
- Antibacterial Substances Produced by Enterococcus faecium
- Effects of Meat-conditioning and Lactic Fermentation on Pork Muscle Protein Degradation
- Isolation of Enterococcus faecium with Antibacterial Activity and Characterization of Its Bacteriocin
- Association Between Serum Uric Acid Levels and Cardiometabolic Risk Factors Among Japanese Junior High School Students
- Morphological Evolution of the InGaN-Based Quantum Well Surface due to a Reduced Density of Threading Dislocations in the Underlying GaN through Higher Growth Pressure
- Optical Properties of GaN Thin Films on Sapphire Substrates Characterized by Variable-Angle Spectroscopic Ellipsometry
- Increased Size of Open Hexagonally Shaped Pits due to Growth Interruption and Its Influence on InGaN/GaN Quantum-Well Structures Grown by Metalorganic Vapor Phase Epitaxy
- Characterization of Double-Buffer Layers and Its Application for the Metalorganic Vapor Phase Epitaxial Growth of GaN
- Evidence for widespread infection of hepatitis E virus among wild rats in Japan
- Immunochemical and Biochemical Identification of the Rice Seed Protein Encoded by cDNA Clone A3-12
- Four Rice Seed cDNA Clones Belonging to the α-Amylase/Trypsin Inhibitor Gene Family Encode Potential Rice Allergens
- Characterization of the Bovine κ-Casein Gene Promoter
- Screening of Rice Strains Deficient in 16-kDa Allergenic Protein
- Isolation and Culture of Bovine Mammary Epithelial Cells and Establishment of Gene Transfection Conditions in the Cells
- Suppressive expression of the glutelin A1-collagen peptide fusion gene in hybrid rice lines with the mutant line, Low Glutelin Content-1 (LGC-1)
- Self-assembly of sapovirus recombinant virus-like particles from polyprotein in mammalian cells
- Types of human leukocyte antigen and decrease in HCV core antigen in serum for predicting efficacy of interferon-α in patients with chronic hepatitis C : analysis by a prospective study
- Association analysis of cytotoxic T-lymphocyte antigen 4 gene polymorphisms with primary biliary cirrhosis in Japanese patients
- Efficacy and safety of addition of minor bloodletting (petit phlebotomy) in hepatitis C virus-infected patients receiving regular glycyrrhizin injections
- A case of well-differentiated cholangiolocellular carcinoma visualized with contrast-enhanced ultrasonography using Sonazoid
- Primitive Neuroectodermal Tumor as a Differential Diagnosis of CD56-Positive Tumors in Adults
- Association of cytotoxic T-lymphocyte antigen 4 gene polymorphisms with type 1 autoimmune hepatitis in Japanese
- Efficacy and safety of 6-month iron reduction therapy in patients with hepatitis C virus-related cirrhosis: a pilot study
- Hepatitis B virus RNA is measurable in serum and can be a new marker for monitoring lamivudine therapy
- Hepatic Hydrothorax in the Absence of Ascites Diagnosed by Intraperitoneal Spraying of Indocyanine Green
- Clinical significance of TT virus infection in maintenance hemodialysis patients of an endemic area for hepatitis C infection
- Relationship between hepatitis C virus infection and schistosomal liver disease : not simply an additive effect
- Seroepidemiological study of hepatitis E virus infection in Japan using a newly developed antibody assay
- Accumulation of Maize Response Regulator Proteins in Mesophyll Cells after Cytokinin Treatment(Biochemistry & Molecular Biology)
- Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli
- Involvement of the α2,8-Polysialyltransferases II/STX and IV/PST in the Biosynthesis of Polysialic Acid Chains on the O-Linked Glycoproteins in Rainbow Trout Ovary
- Improvement of Physicochemical and Enzymatic Properties of Bovine Trypsin by Nonenzymatic Glycation
- ISOLATION AND ROLES IN CELL-CELL INTERACTION OF THE MEMBRANE MICRODOMAINS (LIPID RAFTS) OF DEVELOPING EMBRYOS OF MEDAKA(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Insights into the Enzyme-Substrate Interaction in the Norovirus 3C-like Protease
- FUNCTIONS OF THE COMMON GLYCOTOPES OF GLYCOLIPIDS AND GLYCOPROTEINS IN THE MEMBRANE MICRODOMAIN OF SEA URCHIN SPERM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Structural Characterization of the 16-kDa Allergen, RA17,in Rice Seeds. Prediction of the Secondary Structure and Identification of Intramolecular Disulfide Bridges
- High Voltage Insulation Based on Area and Volume Effects of Cryogenic Liquids for Superconducting Power Apparatus (特集 電力・エネルギ-分野における超電導技術)
- Dynamic Characteristics of Liquid Helium Breakdowm Induced by Quench Phenomena of ac Superconducting Wire
- STUDIES ON MYOCARDIAL CONTRACTILITY : maxV_ MEASURED WITH USUAL CARDIAC CATHETERS
- Nuclear Localization of Ca^/Calmodulin-Dependent Protein Kinase IV and Calspermin
- Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells
- 国動協および公私動協において動物実験に用いられたサルおよびブタのHEV感染調査
- An ELISA-Based Assay for Detergent-solubilized Cellular β1,4-Galactosyltransferase Activity. Use of a Polyacrylamide Derivative with GlcNAc-β Side Chains as a Solid Phase Acceptor Substrate
- Present state of hepatitis E virus epidemiology in Tokyo, Japan
- Nonalcoholic fatty liver disease in Japanese junior high school students : its prevalence and relationship to lifestyle habits
- Immunogenic and Allergenic Potentials of Natural and Recombinant Innocuous Proteins
- Enhancing Effect of Malondialdehyde Modification on the Mouse IgE Response to Protein Antigens(Food & Nutrition)
- Beat-to -beat Changes of Right Ventricular Contraction in Clinical Cases
- In Vitro Translation of α-Amylase mRNA from Dry Wheat Embryos
- WENCKEBACH HEART BLOCK DURING ATRIAL PACING
- Immunochemical Studies on Rice Allergenic Proteins(Food & Nutrition)
- HPLC Analysis of Fluorescence-labeled Oligosaccharides Administered into Rat Stomach(Food & Nutrition)
- PROSPECTIVE COMPARISON BETWEEN SEDATED HIGH-DEFINITION ORAL AND UNSEDATED ULTRATHIN TRANSNASAL ESOPHAGOGASTRODUODENOSCOPY IN THE SAME SUBJECTS : PILOT STUDY
- Immunomagnetic Capture RT-PCR for Detection of Norovirus from Foods Implicated in a Foodborne Outbreak
- Expression of Recombinant Capsid Proteins of Chitta Virus, a Genogroup II Norwalk Virus, and Development of an ELISA to Detect the Viral Antigen
- Effect of an Antisense Sequence on Rice Allergen Genes Comprising a Multigene Family
- Decrease in Rice Allergenic Proteins of Polished Rice Grains by Incubating with a Miso Solution
- Absorption, Migration and Kinetics in Peripheral Blood of Orally Administered Ovalbumin in a Mouse Model
- Expression of β-N-Acetylgalactosaminylated N-Linked Sugar Chains Is Associated with Functional Differentiation of Bovine Mammary Gland
- In Vitro and in Vivo Evaluation of the Efficacy of Bovine Colostrum against Human Rotavirus Infection
- Proton/Pyruvate Cotransport into Mesophyll Chloroplasts of C_4 Plants : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- THE PULMONARY ARTERIAL COMPLIANCE IN CLINICAL CASES
- Binding of Norovirus Virus-Like Particles (VLPs) to Human Intestinal Caco-2 Cells and the Suppressive Effect of Pasteurized Bovine Colostrum on This VLP Binding
- Absorption, Migration and Kinetics in Peripheral Blood of Orally Administered Ovalbumin in a Mouse Model
- Down-Regulation of Protein Tyrosine Phosphatase Gene Expression in Lactating Mouse Mammary Gland
- The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis
- Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan
- Accurate and simple method for quantification of hepatic fat content using magnetic resonance imaging : a prospective study in biopsy-proven nonalcoholic fatty liver disease
- Notch 受容体の細胞外領域におけるO-GlcNAc修飾
- Seroepidemiological study of norovirus infection in Aichi Prefecture, Japan
- Mice are Not Susceptible to Hepatitis E Virus Infection(Virology)
- Distinct genotype and antigenicity among genogroup II sapoviruses
- Confocal Laser Scanning Microscope Imaging of a Pattern in Shrinking Gel
- High Fat Feeding of Lactating Mice Causing a Drastic Reduction in Fat and Energy Content in Milk without Affecting the Apparent Growth of Their Pups and the Production of Major Milk Fat Globule Membrane Components MFG-E8 and Butyrophilin
- Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad : Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells
- Clinical significance of immunoglobulin G4-associated autoimmune hepatitis
- Clinical characteristics of de novo nonalcoholic fatty liver disease following pancreaticoduodenectomy
- A Growing Family of Dual Specificity Phosphatases with Low Molecular Masses^1
- Genetic polymorphisms in CTLA4 and SLC4A2 are differentially associated with the pathogenesis of primary biliary cirrhosis in Japanese patients
- Mice are Not Susceptible to Hepatitis E Virus Infection
- Comparative site-directed mutagenesis in the catalytic amino acid triad in calicivirus proteases