Purification and Properties of the Endo-1,4-β-Glucanase III from Ruminococcus albus

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概要

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• Endoglucanase III (EGIII) was purified from Ruminococcus albus culture supernatant. An enzyme having a molecular weight of 53,000 was stabilized by mercaptoethanol and inhibited by sulfhydryl group-blocking reagents, and exhibited its highest CMC-degrading activity at pH 5.7 and 55℃. The enzyme hydrolyzed cellobiose (G2) and cellotriose (G3) only negligibly, but significantly hydrolyzed cellotetraose (G4), cellopentaose (G5) and cellohexaose (G6). The major hydrolysis reactions conducted by the enzyme were G4→2G2,G5→G2+G3,G6→G2+G4 and G6→2G3. The V_<max> values of these reactions increased remarkably while the K_m values decreased significantly with an increase in degree of polymerization of the substrate.

• 社団法人日本生物工学会の論文
• 1992-01-25

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Shimizu Shoichi

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Shimizu S

  Professor Emeritus Nagoya University

• Shiimizu Susumu

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Matsuda T

  Department Of Applied Molecular Biosciences School Of Bioagricultural Sciences Nagoya University

• Matsuda T

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Matsuda Tsukasa

  School Of Agriculture Nagoya University

• WATANABE YUICHI

  Niigata Research Laboratories, Mitsubishi Gas Chemical Company Inc.

• MORIYAMA RYOUICHI

  School of Agriculture, Nagoya University

• SHIMIZU SHOICHI

  Professor Emeritus, Nagoya University

• Matsuda Tsukasa

  Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University

• Shimizu Shoichi

  Daiichi Pharmaceutical Co. Ltd.

• WATANABE Yuichi

  Laboratory of Bioreaction Engineering, School of Agriculture, Nagoya University

• Moriyama Ryouichi

  School Of Agriculture Nagoya University

• Watanabe Yuichi

  Laboratory Of Bioreaction Engineering School Of Agriculture Nagoya University

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