Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• Clostridium paraputrificum M-21 was transformed with several shuttle plasmids constructed for Clostridium acetobutylicum-Escherichia coli and Clostridium perfringens-E. coli by electroporation. The Clostridium stercorarium xylanase gene xyn10B was successfully expressed in C. paraputrificum M-21 and the expressed protein did not suffer from proteolysis by host protease(s). This system will provide us with a genetic tool for genetic and metabolic engineering of this bacterium.

• 社団法人日本生物工学会の論文
• 2003-09-25

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Baba Daisuke

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Morimoto K

  Faculty Of Agriculture Kagawa University

• Karita Shuichi

  Faculty of Bioresources, Mie University

• MORIMOTO Kenji

  Faculty of Bioresources, Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• Morimoto Kenji

  Faculty Of Agriculture Kagawa University

• Morimoto Kenji

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• KAWASE MAMIKO

  Faculty of Bioresources, Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Karita Shuichi

  Faculty Of Bioresources Mie University

• Kawase Mamiko

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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