Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)

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概要

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• A family-4 α-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.

著者

• Kitaoka Motomitsu

  National Food Research Institute, National Agriculture and Food Research Organization

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Sakka Makiko

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• Kimura Tetsuya

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• SAKKA Kazuo

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• Shima M

  Department Of Pediatrics Nara Medical University

• ANGGRAENI Andian

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• RATANAKHAOKCHAI Khanok

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi

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