Purification and Characterization of the Family J Catalytic Domain Derived from the Clostridium thermocellum Endoglucanase CelJ
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概要
- 論文の詳細を見る
The Clostridium thermocellum endoglucanase CelJ contains two different catalytic domains in a polypeptide, i.e., a subfamily E1 catalytic domain and a family J catalytic domain [J Bacteriol., 178, 5732-5740 (1996)],, The family J catalytic domain (CDJ-CelJ) was produced by a recombinant Escherichia coli and purified. The purified CDJ-CelJ gave a single band on SDS-polyacrylamide gel electrophoresis and the molecular weight of this enzyme (60,000) was consistent with the value (60,333) calculated from the DNA sequence. CDJ-CelJ hydrolyzed various cellulosic substrates, xylan, and lichenan but not p-nitropbenyl (PNP)-cellobioside, PNP-glucoside, or PNP-xyloside at all. CDJ-CelJ was active on Avicel, a microcrystalline cellulose, and the specific activity of CDJ-CelJ on Avicel (0.0078U/mg protein) was comparable to that of CelS, which is recognized as the most important catalytic subunit of the C. thermocellum, cellulosome, suggesting that CelJ is also an important catalytic subunit in the cellulosome of this bacterium, in addition to CelS.
- 社団法人日本農芸化学会の論文
- 1997-03-23
著者
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Ohmiya Kunio
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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KARITA Shuichi
Center for Molecular Biology and Genetics Mie University
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Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
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Ahsan Md.
Department Of Bioscience Faculty Of Bioresources Mie University
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Kimura Tetsuya
Department Of Clinical Retrovirology And Infectious Diseases Center For Aids Research Kumamoto Unive
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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AHSAN Mohammad
Department of Bioscience, Faculty of Bioresources, Mie University
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MATSUMOTO Miwako
Department of Bioscience, Faculty of Bioresources, Mie University
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Matsumoto M
Laboratory Of Metabolic Biochemistry Department Of Biochemistry And Biotechnology Faculty Of Agricul
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Matsumoto Miwako
Department Of Bioscience Faculty Of Bioresources Mie University
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Kimura Tetsuya
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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