High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lactis

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概要

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• The putative xyn11A structural gene (BH0899) encoding a family-11 xylanase from alkaliphilic Bacillus halodurans strain C-125 was heterologously expressed in the yeast Kluyveromyces lactis CBS 1065 and secreted to a level of 156 μg/ml under selective culture conditions in shake flasks. The Xyn11A production level in shake flask cultures of K. lactis CBS 1065 was higher than that reported for other xylanase genes placed under the control of the regulated LAC4 promoter on a plasmid containing an entire sequence of pKD1 from Kluyveromyces drosophilarium. Recombinant Xyn11A was highly active over pH range from 3 to 10, with maximal activity around pH 7. The enzyme showed a specific activity of 628 U/mg-protein on birchwood xylan as substrate, but no cellulase or β-xylosidase activity.

• 社団法人 日本農芸化学会の論文
• 2007-03-23

著者

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Sakka Makiko

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• Kimura Tetsuya

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• SAKKA Kazuo

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• Sakka K

  Graduate School Of Bioresources Mie Univ.

• Shima M

  Department Of Pediatrics Nara Medical University

• Kimura Tetsuya

  Department Of Science Kobe University

• Wamalwa Benson

  Analytical Chemistry Lab. Dep. Of Chemistry Univ. Of Nairobi

• Zhao Guiwen

  Department Of Chemistry University Of Science And Technology Of China

• Sakka Makiko

  Graduate School Of Bioresources Mie Univ.

• Shibata M

  Univ. Tokyo Tokyo Jpn

• Karita S

  Graduate School Of Regional Innovation Studies Mie Univ.

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Wamalwa Benson

  Applied Microbiology Laboratory Graduate School Of Bioresources Mie University

• ZHAO Guangshan

  Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University

• SHIUNDU Paul

  Analytical Chemistry Laboratory, Department of Chemistry, University of Nairobi

• Shiundu Paul

  Analytical Chemistry Laboratory Department Of Chemistry University Of Nairobi

• Kitahashi T

  Brain-operative Expression Laboratory Brain Science Institute

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of ScienceOsaka University

• SHIBATA Masaru

  Department of Earth and Space Science Faculty of Science, Osaka University

• SHIBATA Masaru

  Department of Earth and Space Science, Faculty of Science Osaka University

• SHIBATA Masaru

  Graduate School of Arts and Sciences, University of Tokyo

• SIBATA Masaru

  Department of Physics, University of Illinois:Department of Earth and Space Science, Graduate School of Science, Osaha University

• SHIBATA Masaru

  Department of Earth and Space Science, Osaka University

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