Expression of Hydrogenase Gene in Clostridium paraputrificum for Enhancing Hydrogen Gas Production
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概要
- 論文の詳細を見る
A [Fe] hydrogenase gene (hyd) was cloned from Clostridium paraputrificum in Escherichia coli using a probe of a conserved DNA sequence of clostridia hydrogenase genes amplified by PCR. The hyd consists of an open reading frame of 1, 749-bp, encoding 582 amino acids with a MW of 64, 560. The cloned hyd was ligated into a shuttle vector pJIR751 between E. coli and C. paraputrificum and was sequenced and expressed in C. paraputrificum. The hydrogen gas productivity of the transformant was increased up to 1.7-fold. Since hydrogenase encoded by hyd is the terminal enzyme of the sequential metabolism for hydrogen evolution, fortification of the enzyme in the cell might accelerate hydrogen release.
- 日本マクロエンジニアリング学会の論文
著者
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Morimoto K
Faculty Of Agriculture Kagawa University
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Kimura Tetsuya
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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SAKKA Kazuo
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University
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OHMIYA KUNIO
Applied Microbiology Laboratory, Faculty of Bioresources
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Kimura Tetsuya
Graduate School Of Bioresources Mie University
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MORIMOTO KENJI
Aichi Science and Technology Foundation
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OHMIYA KUNIO
Applied Microbiology, Faculty of Bioresources, Mie University
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KIMURA TETSUYA
Applied Microbiology, Faculty of Bioresources, Mie University
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