Efficient Biosynthesis of D-Allose from D-Psicose by Cross-linked Recombinant L-Rhamnose Isomerase : Separation of Product by Ethanol Crystallization(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• Mass production of a rare aldohexose D-allose from D-psicose was achieved in a batch reaction by crude recombinant L-rhamnose isomerase (L-RhI) cross-linked with glutaraldehyde. The D-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose D-fructose by immobilized recombinant D-tagatose 3-epimerase (D-TE). At an equilibrium state, 25% of D-psicose was isomerized to D-allose, that is, 25g of D-allose was obtained from 100g of D-psicose. The D-allose product was easily separated and crystallized from the reaction mixture that contains 25% D-allose, 8% D-altrose and 67% D-psicose using ethanol. Empirically, approximately 338g, that is, 90% of a theoretical overall yield for the purification of pure D-allose crystals was produced from 1.5kg of D-psicose within 30d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages.

• 社団法人日本生物工学会の論文
• 2006-04-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• OKADA Hiromi

  Department of Hemostasis and Thrombosis, Clinical Research Center, Nagoya Medical Center

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Dep. Of Bioresource Sci. Fac. Of Agriculture Kagawa Univ.

• Izumori Ken

  松谷化学工業 開発研究所

• Izumori Ken

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Leang Khim

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Takada Goro

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takada G

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Granstrorm Tom

  香川大希少糖セ

• Granstroem T

  Rare Sugar Research Center Kagawa University

• Morimoto K

  Faculty Of Agriculture Kagawa University

• Morimoto Kenji

  Department Of Mechanical Systems Engineering Tokyo University Of Agriculture And Technology

• Poonperm Wayoon

  Rare Sugar Research Center, Kagawa University

• Poonperm Wayoon

  Rare Sugar Research Center And Faculty Of Agriculture Kagawa Univeristy

• Morimoto Kenji

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takada Goro

  秋田大学 医学部小児外科学

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Menavuvu Buetusiwa

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Poonperm Wayoon

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Granstrom Tom

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Noguchi Naoki

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Menavuvu Buetusiwa

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Granstrom Tom

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Okada Hiromi

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Noguchi Naoki

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Poonperm Wayoon

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Research Center Kagawa University

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