Production of L-Erythrose via L-Erythrulose from Erythritol Using Microbial and Enzymatic Reactions

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A rare aldotetrose, L-erythrose, was produced from erythritol via a two-atep reaction. In the first step, complete oxidation of erythritol to L-erythrulose was achieved by using Gluconobacter frateurii IFO 3254. Washed cell suspension of the strain grown on tryptic soy broth(TSB)supplemented with 1% D-sorbitol was used to carry out the transformation reaction at 30℃ with shaking at 170rpm. At 10% substrate concentration, 98% erythritol was converted to L-erythrulose within 48h. The produced L-erythrulose was then used as a substrate for the production of L-erythrose. The isomerization of L-erythrulose to L-erythrose was carried out using constitutively produced L-ribose isomerase(L-RI)from the mutant strain Acinetobacter sp. DL-28 grown on D-lyxose mineral salt medium. At equilibrium, the yield of L-erythrose from L-erythrulose was 18% and finally 1.7g L-erythrose was obtained from 10g erythritol. After a number of simple purification steps, the product was isolated from the reaction mixture by ion-exchange column chromatography(Dowex 50W-X2, Ca^<2+>). The structure of the product was determined after NaBH_4 reduction from Infrared(IR)and ^<13>C nuclear magnetic resonance(NMR)spectra.
社団法人日本生物工学会の論文
2001-09-25