Cloning and Sequencing of β-Mannosidase Gene from Aspergillus aculeatus No. F-50

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概要

• 論文の詳細を見る

• The manB gene, coding for a unique β-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli β-galactosidase and the human β-gluclonidase was conserved. This is the first fungal gene that encodes MANB.

• 社団法人日本農芸化学会の論文
• 1999-01-23

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• KAGA Tomoko

  Department of Applied Biological Chemistry, and Research Institute for Advanced Science and Technolo

• SUMITANI Junichi

  Department of Applied Biological Chemistry, and Research Institute for Advanced Science and Technolo

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Kaga Tomoko

  Department Of Applied Biological Chemistry And Research Institute For Advanced Science And Technolog

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