Alteration of Substrate Specificity of Fructosyl-Amino Acid Oxidase from Ulocladium sp. JS-103(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• We showed by random mutagenesis that one-amino-acid substitution at Arg94 in fructosyl-amino acid oxidase from Ulocladium sp. JS-103 enhanced substrate specificity toward fructosyl valine (FV), a model compound of hemoglobin A_<1c>. Kinetic analysis showed that the specificity of the R94W mutant enzyme toward FV was 14-fold that of the wild-type enzyme. The mutant enzyme obtained will be useful in developing an enzymatic measurement method for hemoglobin A_<1c>.

• 社団法人日本生物工学会の論文
• 2006-09-25

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• IMAMURA SHIGEYUKI

  Diagnostic Research and Development Department, Diafnostic Division

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• KOGA Shinji

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• YOSHIOKA Issei

  Diagnostics Research and Development Department, Asahi-Kasei Corporation

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• SUMITANI Jun-ichi

  Department of Applied Biological Chemistry, College of Agriculture, and Research Institute for Advan

• Yoshioka Issei

  Diagnostics R&d Department Fine Chemicals And Diagnostics Division Asahi Kasei Pharma Corporatio

• Yoshioka Issei

  Diagnostics Division Asahi Chemical Industry

• Imamura Shigeyuki

  Diagnostics R&d Department Fine Chemicals And Diagnostics Division Asahi Kasei Pharma Corporatio

• Fujiwara Maki

  Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefe

• Kouzuma Takuji

  Diagnostics R&D Department, Fine Chemicals and Diagnostics Division, Asahi Kasei Pharma Corporation

• Fujiwara Maki

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kouzuma Takuji

  Diagnostics R&d Department Fine Chemicals And Diagnostics Division Asahi Kasei Pharma Corporatio

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Sumitani Jun-ichi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Koga Shinji

  Diagnostics R&d Department Fine Chemicals And Diagnostics Division Asahi Kasei Pharma Corporatio

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

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