Purification and Some Properties of Six Chitinases from Aeromonas sp. No. 10S-24
スポンサーリンク
概要
- 論文の詳細を見る
Six chitinases were purified from a culture supernatant of Aeromonas sp. no. 10S-24 by ammonium sulfate precipitation, DEAE-Sephadex A-50, Butyl-Toyopearl 650M, and chromatofocusing. These enzymes were most active at pH 3.5-4.5 and the optimum temperature were 50℃. The molecular weights of the enzymes were 89,000 to 120,000 from SDS-polyacrylamide gel electrophoresis. N-Terminal amino acid sequences of the enzymes were similar to that of chitinase I.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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UEDA MITSUHIRO
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ueda M
Laboratory Of Biocycle Engineering Department Of Applied Biological Chemistry Osaka Prefecture Unive
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Ueda Mitsuhiro
Laboratory Of Biocycle Engineering Graduate School Of Life And Environmental Sciences Osaka Prefectu
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Fujiwara Ayako
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Fujiwara Ayako
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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Ueda Mitsuhiro
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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