Purification and Propeties of Cellobiose Phosphorylase from Clostridium thermocellum
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概要
- 論文の詳細を見る
Cellobiose phosphorylase was purified 111-fold from a cell extract of Clostridium thermocellum ATCC 27405,with a yield of 31.4%, to electrophoretic and column choromatographic homogeneity. The molecular weight of the enzyme was estimated to be 150,000 by gel filtration and 85,000 by SDS-PAGE, suggesting that it consisted of two identical subunits. It was suggested by spectrophotometric and chemical analyse that the enzyme contained no pyridoxal phosphate. The enzyme was inactivated by N-ethylmaleimide and activated by dithiothreitol, indicating that the exposed thiol group(s) was important for the enzymatic activity. The enzyme could utilize, so far as examined, D-glucose, D-xylose, 2-deoxy-D-glucose, and D-mannose, as acceptors of glucose in the synthetic reaction of disaccharides. The enzyme could to a low degree utilize D-arabinose and D-fucose, as acceptors.
- 社団法人日本生物工学会の論文
- 1995-03-25
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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TANAKA Keijitsu
Department of Bio-resource Science, Faculty of Agriculture, Tottori University
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Tanaka K
Univ. East Asia Yamaguchi Jpn
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Ooi Toshihiko
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ooi Toshihiko
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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IMADA YUKIO
Bio-Center, Research and Development Div., NKK Corporation
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Imada Yukio
Bio-center Research And Development Div. Nkk Corporation
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Tanaka Keijitsu
Department Of Bio-resource Science Faculty Of Agriculture Tottori University
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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