Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
スポンサーリンク
概要
- 論文の詳細を見る
As a step to breed a Saccharomyces cerevisiae strain able to produce ethanol directly from cellulose, we combined cDNA for Aspergillus aculeatus FI-CMCase (FI-carboxymethyl cellulase) with the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter of S. cerevisiae and used the resultant plasmid, pYEC91, to transform S. cerevisiae. The transformed cells produced active FI-CMCase within the cytoplasm. Western-blot analysis following SDS-polyacrylamide gel electrophoresis demonstrated that the cells contained a peptide having the same molecular mass and immunological identity as A. aculeatus FI-CMCase.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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KAWAGUCHI Takashi
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring
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Okada H
Kansai Univ. Osaka Jpn
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Okada H
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Okada H
Nagaoka Univ. Technol. Niigata Jpn
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Okada H
Nagoya City Univ. School Of Medicine Nagoya Jpn
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Okada H
Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy
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Okada Hirosuke
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Okada Hirosuke
Deparment Of Fermentation Technology Osaka University
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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Kawaguchi Takashi
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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Kawaguchi Takashi
College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Drug Safety Research Eisai Co. Ltd.
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Ooi Toshihiko
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ooi Toshihiko
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi T
Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect
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MINAMIGUCHI KAZUHISA
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Minamiguchi Kazuhisa
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawaguchi Takashi
Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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