Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
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概要
- 論文の詳細を見る
We detected a prolyl endopeptidase (EC 3.4.21.26) in the cell-free extract of Pseudomonas sp. KU-22. The enzyme was purified approximately 2,000-fold by a series of column chromatographies. The molecular weight and isoelectric point of the purified enzyme were estimated to be 76,000 and pH 4.9,respectively. The enzyme was most active at 45℃ and pH 8.0 with benzyloxycarbonyl-L-alanyl-L-proline p-nitroanilide (Z-Ala-Ala-Pro-NphNO_2) as a substrate. Significant inhibition of the enzyme by DFP and prolyl endopeptidasespecific inhibitors was observed. Some properties noted, particularly pH stability, amino acid composition, and PI of the enzyme, are clearly different from those of other bacterial prolyl endopeptidases reported to date, indicating that the enzyme is a member of the microbial prolyl endopeptidase family.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
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MIZUKI Eiichi
Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
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Oyama H
Kyoto Inst. Technol. Kyoto Jpn
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Tsuru Daisuke
Department of Applied Microbiology, Kumamoto Institute of Technology
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Mizuki Eiichi
Department Of Applied Genetics And Pest Management Faculty Of Agriculture Kyushu University
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Mizuki Eiichi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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YOSHIMOTO TADASHI
School of Pharmaceutical Sciences, Nagasaki University
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Aoki Hideyuki
Department Of Chemical Engineering Tohoku University
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Yoshimoto T
Nagasaki Univ. Nagasaki Jpn
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasiki University
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Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
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Oyama Hiroshi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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AMANO MAKOTO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Amano Makoto
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Murao S
Sojo Univ. Kumamoto Jpn
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Aoki Hideyuki
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Tsuru D
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Tsuru Daisuke
Department Of Applied Microbial Technology Sojo University
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Tsuru Daisuke
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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Tsuru D
Department Of Applied Microbial Technology Sojo University
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Yoshimoto Tadashi
School of Pharamaceutical Sciences, Nagasaki University
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