Prolylcarboxypeptidase (Angiotensinase C) : Purification and Characterization of the Enzyme from Xanthomonas maltophilia

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概要

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• Prolylcarboxypeptidase (Angiotensinase C, EC 3. 4. 16. 2) was purified to homogeneity from cell free extracts of Xanthomonas maltophilia by ammonium sulfate fractionation and sequential chromatographies on DEAE-Toyopearl, Sephadex G-150, FPLC-Hiload Superdex 200pg, and FPLC-Hitrap SP columns, with an activity recovery of 15%. The molecular weight of the enzyme was found to be 330,000 by gelfiltration and 83,000 by SDS-PAGE, suggesting a tetrameric form for the native enzyme. It had an optimum pH of 8.5 and stability between pH 8.0 and 11.0. The isoelectric point of the enzyme was 6. 6. The enzyme hydrolyzed Pro-X bonds when proline was in the penultimate position from the carboxyl terminal. The enzyme was strongly inhibited by diisopropylfluorophosphate (DFP), while phenylmethylsulfonyl fluoride (PMSF), p-chloromercuribenzoic acid (PCMB), iodoacetamide, and metal chelators had no effect.

• 社団法人日本農芸化学会の論文
• 1995-02-23

著者

• Suga Koushirou

  School of Pharmaceutical Sciences, Nagasaki University

• Ito Kiyoshi

  School of Pharmaceutical Sciences, Nagasaki University

• Tsuru Daisuke

  Department of Applied Microbiology, Kumamoto Institute of Technology

• Ito K

  School Of Pharmaceutical Sciences Nagasaki University

• YOSHIMOTO TADASHI

  School of Pharmaceutical Sciences, Nagasaki University

• Yoshimoto T

  Nagasaki Univ. Nagasaki Jpn

• Yoshlmoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasiki University

• Ito K

  Sapporo Breweries Ltd. Gumma Jpn

• Ito Kiyoshi

  School Of Pharmaceutical Sciences Nagasaki University

• Tsuru D

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Tsuru Daisuke

  Department Of Applied Microbial Technology Sojo University

• Tsuru Daisuke

  Department Of Applied Microbial Technology Kumamoto Institute Of Technology

• Suga Koushirou

  School Of Pharmaceutical Sciences Nagasaki University

• Tsuru D

  Department Of Applied Microbial Technology Sojo University

• Yoshimoto Tomotaka

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

• Ito K

  Yamagata Univ. Yonezawa Jpn

• Yoshimoto Tadashi

  School of Pharamaceutical Sciences, Nagasaki University

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