Purification and Some Properties of the Enzyme Catalyzing the C_γ-Elimination of a Diarylpropane-type Lignin Model from Pseudomonas paucimobilis TMY1009(Biological Chemistry)
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概要
- 論文の詳細を見る
A novel enzyme catalyzing the C_γ-elimination of a diarylpropane-type lignin model, erythro-1,2-bis(4-hydroxy-3-methoxyphenyl)-propane-1,3-diol, was purified from a cell-free extract of Pseudomonas paucimobilis TMY1009. When the diarylpropane was decomposed by the purified enzyme, equimolar amounts of 1,2-bis(4-hydroxy-3-methoxyphenyl)-ethylene and formaldehyde were produced as reaction products, suggesting that the enzymatic reaction is C_γ-deformylation accompanied with dehydroxylation at the C_α-position. Consequently, this enzyme was designated tentatively as diarylpropane formaldehyde lyase. The optimum pH for the enzyme activity was around 7.5 and the Km value for the diarylpropane was 71 μM. This enzyme was seemed to be composed of two identical subunits and the molecular weight of the native enzyme was estimated to be 80,000.
- 社団法人日本農芸化学会の論文
- 1991-05-23
著者
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Habu Naoto
Department of Bioproductive Science, Faculty of Agriculture, Utsunomiya University
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Samejima Masahiro
Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The Universi
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Yoshlmoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University
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Yoshimoto Tadashi
School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R
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Kishi Katsuyuki
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Samejima Masahiro
Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T
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Habu Naoto
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Habu Naoto
Department Of Bioproductive Science Faculty Of Agriculture Utsunomiya University
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Samejima M
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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YOSHIMOTO Tomotaka
Department of Forest Products, Faculty of Agriculture, The University of Tokyo
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Yoshimoto Tomotaka
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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