Chemical Modification of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Tyrosyl Residues Iodinated(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• The neutral protease of Bacillus subtilis var. amylosacchariticus (B. amylosacchariticus) was iodinated with a 25-fold molar excess of iodine at pH 9.4 for 3 min at 0℃, by which treatment the proteolytic activity toward casein was markedly reduced, while the hydrolytic activity toward an N-blocked peptide substrate was rather increased. The modified enzyme was digested with Staphylococcus aureus V8 protease at pH 8.0 and the amino acid sequences of resultant peptides were compared with those obtained from the native enzyme. One of the peptides was found to have an amino acid sequence of Thr-Ala-Asn-Leu-Ile-Tyr-Glu, which corresponds to residue Nos. 153-159 of the enzyme, where Tyr-158 was identified to be mono-iodotyrosine. The other two peptides were those containing Tyr-21 which was mono- and di-iodinated, respectively. Referring to nitration experiments on the neutral protease and the active site structure of thermolysin, it was concluded that the iodination of Tyr-158 is mainly responsible for the activity changes of B. amylosacchariticus neutral protease.

• 社団法人日本農芸化学会の論文
• 1991-11-23

著者

• Kanatani Akio

  School Of Pharmaceutical Sciences Nagasaki University

• YOSHIMOTO TADASHI

  School of Pharmaceutical Sciences, Nagasaki University

• TSURU DAISUKE

  School of Pharmaceutical Sciences, Nagasaki University

• Yoshimoto T

  Nagasaki Univ. Nagasaki Jpn

• Yoshlmoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Yoshimoto Tadashi

  School Of Pharmaceutical Sciences Nagasiki University

• MORIKAWA Shin-ya

  School of Pharmaceutical Sciences, Nagasaki University

• Murao S

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Murao S

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• KOBAYASHI Ryuji

  School of Pharmaceutical Sciences, Nagasaki University

• Morikawa Shin-ya

  School Of Pharmaceutical Sciences Nagasaki University

• Kobayashi Ryuji

  School Of Pharmaceutical Sciences Nagasaki University

• Kobayashi Ryuji

  School Of Health Science Kibi International University

• Murao S

  Sojo Univ. Kumamoto Jpn

• Tsuru D

  School Of Pharmaceutical Sciences Nagasaki University:yakult Central Institute For Microbiological R

• Tsuru Daisuke

  Department Of Applied Microbial Technology Sojo University

• Tsuru D

  Department Of Applied Microbial Technology Sojo University

• Tsuru Daisuke

  School Of Pharmaceutical Sciences Nagasaki University

• Yoshimoto Tomotaka

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

• Yoshimoto Tadashi

  School of Pharamaceutical Sciences, Nagasaki University

関連論文

• 2A16-4 epsilon-Poly-L-lysine(PL)分解酵素遺伝子の取得と破壊株を用いた機能解析(遺伝子工学・核酸工学,一般講演)
• 3P-1072 エナンチオ選択的イミン還元酵素の探索と特徴解析(2a酵素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
• Prolidase from Xanthomonas maltophilia : Purification and Characterization of the Enzyme
• Nucleotide Sequence of the Neutral Protease Gene from Bacillus subtilis var. amylosacchariticus
• Glutathione-independent Formaldehyde Dehydrogenase from Pseudomons putida : Survey of Functional Groups with Special Regard for Cysteine Residues
• Prolylcarboxypeptidase (Angiotensinase C) : Purification and Characterization of the Enzyme from Xanthomonas maltophilia
• Degradation of Streptomyces Metalloprotease Inhibitor (SMPI) by Neutral Protease from Bacillus subtilis var. amylosacchariticus
• CPT-11 Converting Enzyme from Rat Serum : Purification and Some Properties
• NOVEL AND POTENT INHIBITORS SPECIFIC FOR PROLYL ENDOPEPTIDASE AND THEIR ANTI-AMNESIC EFFECT
• SPECIFIC INHIBITORS FOR PROLYL ENDOPEPTIDASE AND THEIR ANTI-AMNESIC EFFECT
• Specific Inhibition of Dipeptidyl Aminopeptidase IV by a New Synthetic Inhibitor, L-Thioprolylthiazolidine(Biological Chemistry)
• Structure Activity Relationship of Inhibitors Specific for Prolyl Endopeptidase(Biological Chemistry)
• Purification and Characterization of Proline Iminopeptidase from Lyophyllum cinerascens
• 516 Studies on Prolyl Endopeptidase from Shakashimeji (Lyophyllum Cinerascens) : Purification and Enzymatic Properties.
• Effects of an 'enrichment feeder' on behavior and feather conditions of caged laying hens
• Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
• 2Fp04 シアノヒドリンに作用するニトリラーゼの特性(酵素学,酵素工学,タンパク質工学,一般講演)
• 2Fp03 微生物による1,3-アダマンタンジオールの位置選択的水酸化(酵素学,酵素工学,タンパク質工学,一般講演)
• 2Fp05 Fusarium属カビのニトリラーゼの分子特性の解析(酵素学,酵素工学,タンパク質工学,一般講演)
• 1Fp09 4-ヒドロキシ安息香酸脱炭酸酵素の構造と機能発現の相関(酵素学,酵素工学,タンパク質工学,一般講演)
• 2B15-2 マンデロニトリルに作用する立体選択的ニトリラーゼ(酵素学・酵素工学・タンパク質工学,一般講演)
• 1F15-2 イソオイゲノール分解酵素の遺伝子クローニングと一次構造解析(酵素学,酵素工学,タンパク質工学,一般講演)
• 1F15-3 二相系におけるイソオイゲノールのバニリンへの微生物変換(酵素学,酵素工学,タンパク質工学,一般講演)
• 可逆的脱炭酸酵素の特性解析と芳香族カルボン酸の合成について
• ACTIVE SITE STRUCTURE AND FUNCTION OF NEUTRAL PROTEASE FROM BACILLUS SUBTILIS var. AMYLOSACCHARITICUS
• Chemical Modification of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Tyrosyl Residues Iodinated(Biological Chemistry)
• Dye-sensitized Photooxidation of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Histidine Residue Oxidized(Biological Chemistry)
• A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
• Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
• A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
• PURIFICATION AND CHARACTERIZATION OF NEW ENZYMES, URETHANE HYDROLASES AND THEIR UTILITY
• New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
• Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469
• Isolation and Characterization of N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338(Biological Chemistry)
• Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
• A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
• Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
• Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
• Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
• A Novel Type of Proteinase, Masikinolysin, from Streptomyces griseoloalbus SN-22
• Isolation and Characterization of a Dipeptidyl Aminopeptidase from Streptomyces sp.WM-23
• Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
• Enhancement of Activities of Cellulases under High Hydrostatic Pressure
• Isolation and Identification of a Pyrogallol Oxidase Producing Microorganism and the Enzyme Production
• Substrate Recognition Mechanism of Proly Aminopeptidase from Serratia marcescens^1
• Glutamate 83 Is Important for Stabilization of Domain-Domain Conformation of Thermus aquaticus Glycerol Kinase
• Crystal Structure of Prolyl Aminopeptidase from Serratia marcescens
• Cloning, Sequencing, High Expression, and Crystallization of the Thermophile Thermus aquaticus Glycerol Kinase
• Roles of the Ser146, Tyr159, and Lys163 Residues in the Catalytic Action of 7α-Hydroxysteroid Dehydrogenase from Escherichia coli
• Cloning and Expression of the cDNA Encoding Prolyl Oligopeptidase (Prolyl Endopeptidase) from Bovine Brain
• Prolyl Aminopeptidase from Serratia marcescens: Cloning of the Enzyme Gene and Crystalliztion of the Expressed Enzyme^1
• Purification and Characterization of Thermostable Glycerol Kinase from Thermus flavus
• Dipeptidyl Peptidase IV from Xanthomonas maltophilia: Sequencing and Expression of the Enzyme Gene and Charactrization of the Expressed Enzyme
• Prolyl Aminopeptidase Is Also Present in Enterobacteriaceae: Cloning and Sequencing of the Hafnia alvei Enzyme-Gene and Characterization of the Expressed Enzyme
• Protease II from Moraxella lacunata: Cloning, Sequencing, and Expression of the Enzyme Gene, and Crystallization of the Expressed Enzyme
• Purification and Properties of Three Xylanases from Aspergillus aculeatus
• Novel Aminopeptidase Specific for Glycine from Actinomucor elegans(Biochemistry & Molecular Biology)
• Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
• Transformation of 7-(4-Hydroxyphenylacetamido)cephalosporanic Acid into a New Cephalosporin Antibiotic, 7-[1-Oxaspiro(2.5)octa-6-oxo-4, 7-diene-2-carboxamido]ceph-alosporanic Acid, by Laccase
• Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid
• Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
• Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
• Comparative Study on the Specificities of Several Fungal Aspartic and Acidic Proteinases towards the Tetradecapeptide of a Renin Substrate(Biological Chemistry)
• Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
• Purification and Characterization of a Novel Type of Chitinase from Vibrio alginolyticus TK-22
• Prolyl Endopeptidase Inhibitors Derived from Actinomycetes
• Secretion of Escherichia coli Aminopeptidase P in Bacillus subtilis Using the Prepro-Structure Coding Region of Subtilisin Amylosacchariticus
• Novel Prolyl Tri/Tetra-Peptidyl Aminopeptidase from Streptomyces mobaraensis : Substrate Specificity and Enzyme Gene Cloning
• CLONING OF AMINOPEPTIDASES P AND N GENES AND CHARACTERIZATION OF EXPRESSED ENZYMES
• Inactivation of Scytatidium lignicolum Acid Protease B with 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propanet(Biological Chemistry)
• Complete Amino Acid Sequence of Neutral Protease from Bacillus subtilis var. amylosacchariticus(Biological Chemistry)
• Inhibition of Scytalidium lignicolum Acid Protease B by 1-Diazo-3-phenyl-2-propanone(Biological Chemistry)
• Directed Evolution to Improve the Thermostability of Prolyl Endopeptidase^1
• Purification and Some Properties of the Enzyme Catalyzing the C_γ-Elimination of a Diarylpropane-type Lignin Model from Pseudomonas paucimobilis TMY1009(Biological Chemistry)
• Purification and Some Properties of Lignostilbene-α,β-dioxygenase Responsible for the C_α-C_β Cleavage of a Diarylpropane Type Lignin Model Compound from Pseudomonas sp. TMY1009(Biological Chemistry)
• A Novel Assay Method for Aminopeptidase P and Partial Purification of Two Types of the Enzyme in Escherichia coli(Biological Chemistry)
• An Aminopeptidase N from Escherichia coli HB101: Purification and Demonstration that the Enzyme Possesses Arylamidase and Peptidase Activitiest(Biological Chemistry)
• 406 Comparison of Prolyl Endopeptidases Produced in Culture Medium and Fruit-body of Agaricus bisporus (Mushroom)
• 有機溶媒耐性酵素を活用したケミカル生産(疎水性ケミカルのバイオプロダクションを目指した基盤研究)
• Behavioral Responses of Japanese Wild Boars to the Person in Attendance during the Pre- and Post-farrowing Periods under Captive Conditions
• Behavioral Responses of Japanese Wild Boars to the Person in Attendance during the Pre- and Post-farrowing Periods under Captive Conditions
• Color Discrimination in Dogs
• Color Discrimination in Dogs
• Behavior of Japanese Wild Boars, Sus Scrofa leucomystax, during the First Week after Parturition in Farrowing Pens
• Behavior of Japanese Wild Boars, Sus Scrofa leucomystax, during the First Week after Parturition in Farrowing Pens
• Courtship Behavior of Japanese Wild Boars, Sus scrofa leucomystax, under Captive Conditions
• Courtship Behavior of Japanese Wild Boars, Sus scrofa leucomystax, under Captive Conditions
• Purification and Enzymatic Properties of an Endo-α-N-Acetyl-Galactosaminidase from Bacillus laterosporus SS-16
• Are Litter Mates Needed for Each Piglet to Keep its Exclusive Teat after the Establishment of a Teat Order?
• 2Da05 アミノ基転移酵素による光学活性アミンの合成(酵素学・酵素工学,一般講演)
• 微生物の有機溶媒耐性酵素とそのケミカル生産への活用
• Cloning of L-Amino Acid Deaminase Gene from Proteus vulgaris
• 2Da04 酢酸菌による1,3-プロパンジオール類からアクリル酸類への変換(酵素学・酵素工学,一般講演)
• Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins
• 2Cp23 Rhodococcus rhodochrous J1ニトリラーゼによるマロノニトリル誘導体の立体選択的加水分解(酵素学,酵素工学,一般講演)
• 2Cp22 (S)-イミン還元酵素による光学活性アミン合成(酵素学,酵素工学,一般講演)
• 2P-042 様々なイミンに作用する(R)-イミン還元酵素の特徴解析(酵素学,酵素工学,一般講演)
• 2P-041 1-アセチル-2-メチルピロリジンを立体選択的に加水分解するハイドロラーゼの特徴解析(酵素学,酵素工学,一般講演)
• 2P-039 1,4-ジアセチル-2-メチルピペラジンに作用する立体特異的ハイドロラーゼの探索(酵素学,酵素工学,一般講演)
• Prolyl Aminopeptidase Is Also Present in Enterobacteriaceae: Cloning and Sequencing of the Hafnia alvei Enzyme-Gene and Characterization of the Expressed Enzyme.

もっと見る 閉じる

スポンサーリンク