Purification and Properties of Three Xylanases from Aspergillus aculeatus
スポンサーリンク
概要
- 論文の詳細を見る
Three distinct extracellular xylanases (FIa-, FIb-, and FIII-xylanases) from a culture filtrate of Aspergillus aculeatus No. F-50 were purified to homogeneity by SDS polyacrylamide gel electrophoresis. The molecular weights of FIa-, FIb-, and FIII-xylanases were estimated to be 18,000, 26,000, and 52,000, and their pI values were pH 5.6, 9.0, and 3.8, respectively. The pH optima of xylanase activities were from 4.5 to 5.0. The optimum temperatures for enzyme activities were from 50℃ to 70℃. All three xylanases were highly specific for xylan hydrolysis, and they did not cleave xylobiose.
- 社団法人日本農芸化学会の論文
- 1995-03-23
著者
-
FUJIMOTO Hiroaki
Department of Urology, Niigata University School of Medicine
-
Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Murao S
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
-
Murao S
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
Takizawa T
Meiji Seika Kaisha Ltd. Saitama Jpn
-
Takizawa Toshio
Bioscience Laboratories Meiji Seika Kaisha Ltd.
-
Morikawa Shin-ya
School Of Pharmaceutical Sciences Nagasaki University
-
Ooi Toshihiko
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
-
Wang San-Lang
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
-
Hidaka Hidemasa
Bioscience Laboratories, Meiji Seika Kaisha, Ltd.
-
Wang San-lang
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
Arai M
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
-
Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Murao S
Sojo Univ. Kumamoto Jpn
-
Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Ooi Toshihiko
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
Hidaka H
Nagasaki Univ. Nagasaki Jpn
-
Wang San-lang
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Fujimoto Hiroaki
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
-
MURAO SAWAO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
関連論文
- Retroperitoneoscopy-assisted renal biopsy for pediatric patients : Comparison withretroperitoneoscopic renal biopsy
- Degradation of Streptomyces Metalloprotease Inhibitor (SMPI) by Neutral Protease from Bacillus subtilis var. amylosacchariticus
- Importance of the Ketone Function for the Phytotoxicity of Spiciferone A, a Phytotoxin Produced by the Fungus Cochliobolus spicifer
- Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
- Isolation and Biological Activities of N^-Desmethyl Analogus of Tumor Promoter Teleocidin from Streptoverticillium blastmyceticum(Organic Chemistry)
- Structure and Tumor-promoting Activity of New Teleocidin-related Metabolites (Blastmycetins) from Streptoverticillium blastmyceticum(Organic Chemistry)
- New Teleocidin-related Metabolites from Streptoverticillium blastmyceticum Producing Tumor-promoting Indole Alkaloids(Organic Chemistry)
- Substitution Reaction on the Indole Ring of (-)-Indolactam V, the Fundamental Structure of Teleocidins
- Expression of a Gene Encoding Chitinase(pCA 8 ORF)from Aeromonas sp.No.10S-24 in Escherichia coli and Enzyme Characterization
- Identification of the Microorganism Which Produces Epstein-Barr Virus Early Antigen-inducing Indole Alkaloids
- ACTIVE SITE STRUCTURE AND FUNCTION OF NEUTRAL PROTEASE FROM BACILLUS SUBTILIS var. AMYLOSACCHARITICUS
- Chemical Modification of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Tyrosyl Residues Iodinated(Biological Chemistry)
- Dye-sensitized Photooxidation of Neutral Protease from Bacillus subtilis var. amylosacchariticus : Assignment of Histidine Residue Oxidized(Biological Chemistry)
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Mycelium of Lentinus edodes
- A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- PURIFICATION AND CHARACTERIZATION OF NEW ENZYMES, URETHANE HYDROLASES AND THEIR UTILITY
- New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
- A New Screening Method for Microorganisms Which Produce Carboxypeptidases
- New Enzymatic Colorimetric Reactions of Benzoic Acid Derivatives with ABTS [2, 2-Azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] in the Presence of Laccase
- Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469
- Isolation and Characterization of N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338(Biological Chemistry)
- Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
- A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
- Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
- Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
- Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
- A Novel Type of Proteinase, Masikinolysin, from Streptomyces griseoloalbus SN-22
- Isolation and Characterization of a Dipeptidyl Aminopeptidase from Streptomyces sp.WM-23
- Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
- Enhancement of Activities of Cellulases under High Hydrostatic Pressure
- Isolation and Identification of a Pyrogallol Oxidase Producing Microorganism and the Enzyme Production
- Purification and Properties of Three Xylanases from Aspergillus aculeatus
- Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
- Transformation of 7-(4-Hydroxyphenylacetamido)cephalosporanic Acid into a New Cephalosporin Antibiotic, 7-[1-Oxaspiro(2.5)octa-6-oxo-4, 7-diene-2-carboxamido]ceph-alosporanic Acid, by Laccase
- Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid
- Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
- Additional Evidence for the Identity of Scytalidium lignicolum Acid Proteinases with the Carboxyl Proteinase Group : The Interaction between Angiotensin I and S-PI-Insensitive Acid Proteinases by Means of A Zinc(II)-Dye Complex as a Probe(Biological Chemi
- Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
- Comparative Study on the Specificities of Several Fungal Aspartic and Acidic Proteinases towards the Tetradecapeptide of a Renin Substrate(Biological Chemistry)
- Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
- New Oleanane Triterpene with Three Ketones Produced by Penicillium simplicissimum ATCC 90288
- Purification and Characterization of a Novel Type of Chitinase from Vibrio alginolyticus TK-22
- Purification and Characterization of an Alkaline Proteinase Produced by Pimelobacter sp. Z-483
- Isolation of Propioxatin A from Actinosynnema sp. SI-23 during a Screening for Serratia piscatorum Metalloproteinase Inhibitors
- Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
- Cloning, Nucleotide Sequence and Expression of the β-N-Acetyl-glucosaminidase Gene from Aeromonas sp.No.10S-24
- Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1, 3-Mannanase Activity
- Identification of Microorganisms in Traditional Asian Foods Made with Fermented Wheat Flour and Their Hypoallergenization
- Interaction of Proteinaceous Protease Inhibitor of Bacillus subtilis with Intracellular Proteases from the Same Strain(Microbiology & Fermentation Industry)
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Properties of Ascorbate Oxidase Produced by Acremonium sp. HI-25
- Isolation and Purification of Ascorbate Oxidase from Acremonium sp.HI-25
- An Acidic Ascorbate Oxidase Produced by Acremonium sp. HI-25(Biological Chemistry)
- Inactivation of Scytatidium lignicolum Acid Protease B with 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propanet(Biological Chemistry)
- Structures and Activity of Cellulase Inhibitors Enzymatically Synthesized from Cello-oligosaccharides and 1-Deoxynojirimycin
- Cloning and Sequencing of an Endoglucanase Gene from Scopulariopsis brevicaulis TOF-1212,and Its Expression in Saccharomyces cerevisiae
- Different Action by Two Avicelases from Aspergillus aculeatus(Biological Chemistry)
- Reduced Cellulose as a Substrate of Cellulases(Biological Chemistry)
- Enzymatic Properties of Hydrocellulase from Aspergillus aculeatus
- A Novel Thermostable, S-PI(Pepstatin Ac)-insensitive Acid Proteinase from Thermophilic Bacillus novosp. Strain MN-32(Biological Chemistry)
- Cloning and Sequence Analysis of a Chitinase Gene(pCA8 ORF)from Aeromonas sp.No.10S-24
- Expression of the Chitinase III Gene of Aeromonas sp. No. 10S-24 in Escherichia coli
- Purification and Some Properties of Six Chitinases from Aeromonas sp. No. 10S-24
- Molecular Cloning and Nucleotide Sequence of the Gene Encoding Chitinase II from Aeromonas sp. No. 10S-24
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Purification and Propeties of Cellobiose Phosphorylase from Clostridium thermocellum
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- Effects of α- and β-Arbutin on Activity of Tyrosinases from Mushroom and Mouse Melanoma
- Colorimetric Measurement of Total Activity of Leucine Aminopeptidase and Arylamidase in Serum Using Laccase-Catalyzed Oxidative Decarboxylation
- A New Microorganism Producing a Glucosyl Transfer Enzyme to Polyphenols
- Purification and Some Properties of a Thermostable Metal Proteinase Produced by Thermomicrobium sp. KN-22 Strain(Biological Chemistry)
- New Proteinacens α-Amylase inhibitor (T-76) from Streptomyces nitrosporeus
- Purification and Some Properties of Chitinases from Aeromonas sp. No. 10S-24
- Studies on β-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin : Isolation and Characterization of an Isozyme
- Enzymatic Properties of Two Avicelases from Aspergillus aculeatus No. F-50(Biological Chemistry)
- New Okaramine Congeners, Okaramines D, E, and F, from Penicillium simplicissimum ATCC 90288
- A Novel Pyrazine Compound Produced from Chitin by the Activity of the Enzyme from Vibrio alginolyticus TK-24
- Alteration of Substrate Specificity of Fructosyl-Amino Acid Oxidase from Ulocladium sp. JS-103(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- The Conserved Tryptophan-Arginine-Tyrosine Motif of a Proteinaceous α-Amylase Inhibitor T-76 from Streptomyces nitrosporeus Is Important for Inhibition of Animal α-Amylases but not for an α-Amylase from Bacillus sp.No.195
- Cloning and Sequencing of β-Mannosidase Gene from Aspergillus aculeatus No. F-50
- Expression of Aspergillus aculeatus No. F-50 Cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae
- Bacillus Animal Type α-Amylase : Cloning and Sequencing of the Gene, and Comparison of the Deduced Amino Acid Sequence with That of Other Amylases
- Cloning, Nucleotide Sequence, and Expression of the Clostridium thermocellum Cellodextrin Phosphorylase Gene and Its Application to Synthesis of Cellulase Inhibitors
- Cloning, Nucleotide Sequence, and Transcriptional Analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase I(cbhI)Gene
- Enzymatic Synthesis of (+)Catechin-α-glucoside and Its Effect on Tyrosinase Activity
- Molecular Cloning and Expression of Proteinaceous α-Amylase Inhibitor Gene from Streptomyces nitrosporeus in Escherichia coli
- Purification and Some Properties of a Haim-sensitive α-Amylase from Newly Isolated Bacillus sp. No. 195
- Okaramine C, a New Insecticidal Indole Alkaloid from Penicillium simplicissimum(Organic Chemistry)
- Identification of the Microorganism Which Produces Insecticidal Indole Alkaloids, Okaramines A and B(Microbiology & Fermentation Industry)
- Inhibition of Lysozyme Activity by Acidic Polymers(Biological Chemistry)
- Isolation and Characterization of a Novel Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
- Identification of a Bacterium which Produced Cell Wall Lytic Enzyme for Red Yeast and Purification of the Enzyme : Studies on Red Yeast Cell Walls(V)
- Structure of Fucogalactomannan of Red Yeast Cell Wall
- Inhibition of Sporulation of Bacillus subtilis by MAPI, a Serine Protease Inhibitor, and Interaction of MAPI with Membrane Bound Protease
- Isolation and Identification of a Hog Pancreatic α-Amylase Inhibitor (Haim) Producing Streptomyces