Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
A S-PI(Pepstatin Ac)-insensitive carboxyl proteinase was found in culture filtrate of a Xanthomonas sp, bacterium. The carboxyl proteinase was highly purified and about 100 mg of the enzyme was obtained from 601 of culture filtrate, with a recovery of 25%. The optimum condition for the action of the purified enzyme toward casein was approx, pH 2.7 and its activity was not inhibited by any of such carboxyl proteinase inhibitors as Pepstatin, S-PI, and DAN but EPNP inhibited it. Such behavior of the enzyme against inhibitors resembles that of Pseudomonas sp, carboxyl proteinase, the first found from a bacterium. Some differences were observed, however, in their properties such as optimum pH, isoelectric point, and amino acid composition.
- 社団法人日本農芸化学会の論文
- 1987-11-23
著者
-
TERASHITA Takao
Department of Food and Nutrition, Faculty of Agriculture, Kinki University
-
Suzuki Ken-Ichiro
Japan Collection of Microorganisms, RIKEN
-
Murao Sawao
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Suzuki Ken-ichiro
Japan Collection Of Microorganisms Riken Institute Of Physical And Chemical Research
-
Suzuki Ken-ichiro
Japan Collection Of Microorganisms Riken
-
Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture:(present
-
ODA Kohe
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
NAKAZIMA Takanori
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Oda Kohe
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Terashita Takao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Nakazima Takanori
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
関連論文
- A possible role of the key enzymes of the glyoxylate and gluconeogenesis pathways for fruit-body formation of the wood-rotting basidiomycete Flammulina velutipes
- 316 Phylogenetic Analysis of Coryneform and Nocardioform bacteria by 5S rRNA Sequences.
- 101 Identification of Lactobacillus plantarum and Lactobacillus pentosus by photobiotin labeling DNA-DNA hybridization
- Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
- Horseradish Peroxidase Degrades Lipid Hydroperoxides and Suppresses Lipid Peroxidation of Polyunsaturated Fatty Acids in the Presence of Phenolic Antioxidants(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Identification of the Microorganism Which Produces Epstein-Barr Virus Early Antigen-inducing Indole Alkaloids
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- Streptomyces Pepsin Inhibitor-Insensitive Carboxyl Proteinase from Lentinus edodes
- Purification and Some Properties of Carboxyl Proteinase in Mycelium of Lentinus edodes
- Molecular Cloning of the Gene Encoding Thermostable Endo-1,5-α-L-arabinase of Bacillus thermodenitrificans TS-3 and Its Expression in Bacillus subtilis(Biochemistry & Molecular Biology)
- Molecular Cloning, DNA Sequence, and Expression of the Gene Encoding for Thermostable Pectate Lyase of Thermophilic Bacillus sp. TS 47
- Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
- Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
- A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
- A New Screening Method for Microorganisms Which Produce Carboxypeptidases
- New Enzymatic Colorimetric Reactions of Benzoic Acid Derivatives with ABTS [2, 2-Azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] in the Presence of Laccase
- Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469
- Isolation and Characterization of N^α-Benzyloxycarbonyl Amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338(Biological Chemistry)
- Structures of the Laccase-catalyzed Oxidation Products of Hydroxy-benzoic Acids in the Presence of ABTS [2,2'-Azino-di-(3-ethylbenzothiazoline-6-sulfonic Acid)]
- A Simultaneous Assay Method for L-Glutamate and L-Pyroglutamate Contents in Soy Sauce Using a 5-Oxoprolinase (without ATP Hydrolyzing Activity)
- Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum
- Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp. KU-22
- Isolation and Characterization of a Novel 5-Oxoprolinase (without ATP-hydrolyzing)from Alcaligenes faecalis N-38A
- Isolation and Characterization of a Dipeptidyl Aminopeptidase from Streptomyces sp.WM-23
- Purification and Characterization of Arctium lappa L. (Edible Burdock) Polyphenol Oxidase
- Enhancement of Activities of Cellulases under High Hydrostatic Pressure
- Isolation and Identification of a Pyrogallol Oxidase Producing Microorganism and the Enzyme Production
- Localization of Glucomannan and Fucogalactomannan in Rhodotorula glutinis Cell Wall and Spheroplast Formation of Its Living Cell
- Purification and Properties of Three Xylanases from Aspergillus aculeatus
- Oxidative Decarboxylations of 4-Hydroxymandelic Acid and 2-(4-Hydroxyphenyl)glycine by Laccase from Coriolus versicolor and Bilirubin Oxidases from Trachyderma tsunodae and Myrothecium verrucaria
- Transformation of 7-(4-Hydroxyphenylacetamido)cephalosporanic Acid into a New Cephalosporin Antibiotic, 7-[1-Oxaspiro(2.5)octa-6-oxo-4, 7-diene-2-carboxamido]ceph-alosporanic Acid, by Laccase
- Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid
- Isolation and Characterization of a Proteinaceous Protease Inhibitor from Bacillus subtilis(Microbiology & Fermentation Industry)
- Additional Evidence for the Identity of Scytalidium lignicolum Acid Proteinases with the Carboxyl Proteinase Group : The Interaction between Angiotensin I and S-PI-Insensitive Acid Proteinases by Means of A Zinc(II)-Dye Complex as a Probe(Biological Chemi
- Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
- Comparative Study on the Specificities of Several Fungal Aspartic and Acidic Proteinases towards the Tetradecapeptide of a Renin Substrate(Biological Chemistry)
- Carboxyl Proteinase Essential for Maturation of Basidiospores of Lentinus edodes (shiitake)
- Purification and Characterization of a Novel Type of Chitinase from Vibrio alginolyticus TK-22
- Purification and Characterization of an Alkaline Proteinase Produced by Pimelobacter sp. Z-483
- Isolation of Propioxatin A from Actinosynnema sp. SI-23 during a Screening for Serratia piscatorum Metalloproteinase Inhibitors
- Purification and Properties of an S-PI(Pepstatin Ac)-insensitive Carboxyl Proteinase from a Xanthomonas sp. Bacterium(Biological Chemistry)
- Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1, 3-Mannanase Activity
- 213 Chemotaxonomic Characterization of Aerobic, Gram-positive Rods Isolated from Plowed Soils
- Interaction of Proteinaceous Protease Inhibitor of Bacillus subtilis with Intracellular Proteases from the Same Strain(Microbiology & Fermentation Industry)
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Properties of Ascorbate Oxidase Produced by Acremonium sp. HI-25
- Isolation and Purification of Ascorbate Oxidase from Acremonium sp.HI-25
- An Acidic Ascorbate Oxidase Produced by Acremonium sp. HI-25(Biological Chemistry)
- Different Action by Two Avicelases from Aspergillus aculeatus(Biological Chemistry)
- Reduced Cellulose as a Substrate of Cellulases(Biological Chemistry)
- Enzymatic Properties of Hydrocellulase from Aspergillus aculeatus
- A Novel Thermostable, S-PI(Pepstatin Ac)-insensitive Acid Proteinase from Thermophilic Bacillus novosp. Strain MN-32(Biological Chemistry)
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- Effects of α- and β-Arbutin on Activity of Tyrosinases from Mushroom and Mouse Melanoma
- Colorimetric Measurement of Total Activity of Leucine Aminopeptidase and Arylamidase in Serum Using Laccase-Catalyzed Oxidative Decarboxylation
- A New Microorganism Producing a Glucosyl Transfer Enzyme to Polyphenols
- Purification and Some Properties of a Thermostable Metal Proteinase Produced by Thermomicrobium sp. KN-22 Strain(Biological Chemistry)
- New Proteinacens α-Amylase inhibitor (T-76) from Streptomyces nitrosporeus
- Studies on β-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin : Isolation and Characterization of an Isozyme
- Enzymatic Properties of Two Avicelases from Aspergillus aculeatus No. F-50(Biological Chemistry)
- New Okaramine Congeners, Okaramines D, E, and F, from Penicillium simplicissimum ATCC 90288
- A Novel Pyrazine Compound Produced from Chitin by the Activity of the Enzyme from Vibrio alginolyticus TK-24
- Enzymatic Synthesis of (+)Catechin-α-glucoside and Its Effect on Tyrosinase Activity
- Molecular Cloning and Expression of Proteinaceous α-Amylase Inhibitor Gene from Streptomyces nitrosporeus in Escherichia coli
- Purification and Some Properties of a Haim-sensitive α-Amylase from Newly Isolated Bacillus sp. No. 195
- Okaramine C, a New Insecticidal Indole Alkaloid from Penicillium simplicissimum(Organic Chemistry)
- Identification of the Microorganism Which Produces Insecticidal Indole Alkaloids, Okaramines A and B(Microbiology & Fermentation Industry)
- Inhibition of Lysozyme Activity by Acidic Polymers(Biological Chemistry)
- Isolation and Characterization of a Novel Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
- Some Properties of Hen Egg White Lysozyme Inhibitor from Bacillus subtilis I-139(Biological Chemistry)
- Lysozyme Inhibitory Activity of DNA from Bacillus subtilis I-139(Biological Chemistry)
- Okaramine B, an Insecticidal Indole Alkaloid, Produced by Penicillium simplicissimum AK-40(Organic Chemistry)
- Okaramine A, a Novel Indole Alkaloid with Insecticidal Activity, from Penicillium simplicissimum AK-40(Organic Chemistry)
- Isolation and Characterization of Fructokinase from Pseudomonas sp. KN-21
- Isolation and Characterization of a Serine Proteinase, Inactivating m-Subunit of Lactate Dehydrogenase, from Penicillium citrinum KE-1
- Reduction in the Time Required for Sterilization by Use of a Novel Sterilizer with Rotary Vibrators
- Identification of a Bacterium which Produced Cell Wall Lytic Enzyme for Red Yeast and Purification of the Enzyme : Studies on Red Yeast Cell Walls(V)
- A Novel Trehalase Inhibitor, Trehalostatin, Produced byAmycolatopsis trehalostatica HG-25(Microbiology & Fermentation Industry)
- A New Facultative Anaerobic Xylan-using Alkalophile Lacking Cytochrome, Quinone, and Catalase
- Purification and Characterization of an α-Amylase Inhibitor (Haim) Produced by Streptomyces griseosporeus YM-25
- Inhibition of Sporulation of Genus Bacillus by Various Microbial Protease Inhibitors
- Control of Protease Activity during Sporulation of Bacillus subtilis
- Structure of Fucogalactomannan of Red Yeast Cell Wall
- Purification and Some Properties of Bilirubin Oxidase of Myrothecium verrucaria MT-1
- Stimulation of Sporulation of Bacillus subtilis by Adenosine- 5-triphosphate-3- diphosphate (pppApp)
- Enzymatic Cleavage of the Histydyl-Prolyl Bond of Proangiotensin by Carboxyl Proteinases from Aspergillus sojae and Scytalidium lignicolum
- Inhibition of Sporulation of Bacillus subtilis by MAPI, a Serine Protease Inhibitor, and Interaction of MAPI with Membrane Bound Protease
- Purification and Characterization of a Membrane Bound Serine Protease of Bacillus subtilis IFO 3027
- Regulatory Effects of Purine and Pyrimidine Nucleoside 5-Di(tri)-phosphate-3-diphosphates on Eucaryote Protein Synthesis
- Cytosolic Aspartate Aminotransferase Inactivating-enzyme from Streptomyces violaceochromogenes
- Isolation and Characterization of a Novel Enzyme, Nα-Benzyloxycarbonyl Amino Acid Urethane Hydrolase, from Streptococcus faecalis R ATCC 8043
- A Novel Enzyme, Nα-Benzyloxy-carbonyl Amino Acid Urethane Hydrolase IV
- Substrate Specificity and Stoichiometry of Nα-Benzyloxycarbonyl Amino Acid Urethane Hydrolase from Streptococcus faecalis R ATCC 8043
- A New Enzyme, Nα-Benzyloxycarbonyl Moiety Hydrolytic Enzyme, from Streptococcus faecalis R
- Isolation and Structure-activity Relationship of Some Amylostatins (F-lb Fraction) Produced by Streptomyces diastaticus subsp amylostaticus No. 9410