Molecular Cloning of the Gene Encoding Thermostable Endo-1,5-α-L-arabinase of Bacillus thermodenitrificans TS-3 and Its Expression in Bacillus subtilis(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The gene that encodes a thermostable endo-arabinase (called ABN-TS) from Bacillus thermodenitrificans TS-3 was cloned, sequenced, and expressed in the mesophilic B. subtilis. The gene contained an open reading frame consists of 939 bp, which encodes 313 amino acids. The deduced amino acid sequence of the enzyme showed 50, 46, and 36% similarity with endo-arabinase from B. subtilis IFO 3134 (PPase-C), Pseudomonas fluorescens (ArbA), and Aspergillus niger (ABNA), respectively. The hydrophobic and acidic amino acids making up ABN-TS outnumbered those in PPase-C. The gene product expressed in B. subtilis, as the host, had substantially the same characteristics, and was stable up to 70℃, and the reaction was optimal around 70℃, as well as native ABN-TS.
- 社団法人日本農芸化学会の論文
- 2002-02-23
著者
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Yoshikawa K
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa Kentaro
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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TERASHITA Takao
Department of Food and Nutrition, Faculty of Agriculture, Kinki University
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Yoshikawa K
Department Of Bioscience Faculty Of Bioresources Mie University
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TAKAO Makoto
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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YAMAGUCHI Asako
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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YOSHIKAWA Kentaro
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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YAMAGUCHI Asako
Research Institute for Advanced Science and Technology, Osaka Prefecture University
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TAKAO Makoto
IGA Bioresearch
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Takao Makoto
Laboratory Of Food Microbiological Engineering Department Of Food And Nutrition Faculty Of Agricultu
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Takao Makoto
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Terashita T
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Terashita T
Kinki Univ. Nara Jpn
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Terashita Takao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Yamaguchi Asako
Department Of Food Science And Nutrition Faculty Of Agriculture Kinki University
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Yoshikawa Kentaro
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Yoshida Kouji
Department Of Apple Research National Institute Of Fruit Tree Science
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