Purification and Characterization of a Pectin-Releasing Enzyme Produced by Kluyveromyces wickerhamii

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概要

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• A pectin-releasing enzyme produced by Kluyveromyces wickerhamii IFO 1675 (PPase-W) was purified to homogeneity from a culture filtrate by cation-exchange and size-exclusion chromatographies. This enzyme had a molecular weight of 35,000 determined by both size exclusion chromatography and ultracentrifugal analysis, and of 40,000 by SDS-PAGE. It contained 2.4% sugar, and its isoelectric point was at pH5.2. PPase-W catalyzed the release of highly polymerized pectin from various protopectins, and also showed endopolygalacturonase (endo-PGase) activity. The purified enzyme had optimum PGase activity at about pH5.2 and 50℃ and was stable in the range of pH from 4.0 to 7.0 and up to 50℃. THe properties of PPase-W were compared with those of PPase-F from Kluyveromyces fragilis IFO 0288,and some differences were found. Also, some preliminary data dealing with the relationship between enzyme activities (PPase and endo-PGase) and protein structure are discussed.

• 社団法人日本生物工学会の論文
• 1994-04-25

著者

• Hours R

  Univ. Osaka Prefecture Osaka Jpn

• YOSHITAKE SHINOBU

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• NUMATA TAKESHI

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• KATSURAGI TOHORU

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• HOURS ROQUE

  Career Researcher from the Argentine National Research Counci (CONICET), and Fellow of the Japan Soc

• SAKAI TAKUO

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Numata Takeshi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Hours Roque

  Career Researcher From The Argentine National Research Counci (conicet) And Fellow Of The Japan Soci

• Sakai Takuo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Katsuragi Tohoru

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Yoshitake Shinobu

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

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