Cloning and Expression in Escherichia coli of Sucrose Phosphorylase Gene from Leuconostoc mesenteroides No. 165
スポンサーリンク
概要
- 論文の詳細を見る
The sucrose phosphorylase gene of an isolate, Leuconostoc mesenteroides No. 165, was amplified by PCR, cloned on pUC118, and expressed in E. coli. The nucleotide sequence of the gene showed 96.3% similarity to that of L. mesenteroides ATCC12291 and 67% to that of Streptococcus mutans, but low similarity to the Agrobacterium vitis gene. The cloned gene, which fusing with lacZα, was expressed inducibly with IPTG in E. coli to produce an active enzyme in large quantities that accounted for aboiit 50% of the total cell protein.
- 社団法人日本農芸化学会の論文
- 1996-02-23
著者
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KAWASAKI Haruhiko
Department of Agricultural Chemistry, University of Osaka Prefecture
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture University Of Osaka Prefecture
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Kawasaki H
Department Of Environmental Materials Science Tokyo Denki University
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NAKAMURA Narutoshi
Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University
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OHMORI Masaaki
Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University
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Ohmori Masaaki
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Nakamura N
Kansai Univ. Osaka Jpn
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NAKAMURA Narutoshi
Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture Univeisity
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