Chromosomal Deletion or Rearrangement in Chimeric Hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus Obtained by Cell Fusion

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概要

• 論文の詳細を見る

• We isolated three hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus, a species that secretes glucoamylase and that has been reclassified recently as a strain of Saccharoamyces cerevisiae, for yeast protoplast fusion, and characterized their chromosomes. When the chromosomes of the hybrids were analyzed by electrophoresis in a contour-clamped homogeneous electric field, their migration patterns were similar to those of the parental strains but with slight differences between hybrids. Southern hybridization using the α-amylase and glucoamylase genes from S. fibuligera and S. diastaticus, respectively, as probes showed that these genes were duplicated or deleted in the hybrids. These results demonstrated that the hybrids contained chimeric chromosomes as a result of recombination or rearrangement.

• 社団法人日本生物工学会の論文
• 1996-04-25

著者

• Kishida Masao

  Center For Research And Development Of Bioresources University Of Osaka Prefecture

• Kishida Masao

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University

• Kishida M

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University

• SAKAI TAKUO

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• MUGURUMA TOMOAKI

  Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University

• SAKANAKA KAZUNOBU

  (Present address)Advanced Technology Laboratory, Kubota Corporation

• KATSURAGI TOHORU

  (Present address)Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Kishida Masao

  Mitsubishi Kasei Institute Of Life Sciences

• Kishida Masao

  Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University

• Sakai Takuo

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• Sakai Takuo

  Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture

• Sakai Takuo

  Center For Research And Development Of Bioresources University Of Osaka Prefecture

• Sakai Takuo

  Department Of Food Science Faculty Of Agriculture Kinki University

• Sakai Takuo

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• Sakai Takuo

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity

• Sakai Takuo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Katsuragi Tohoru

  (present Address)graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Sakai T

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• Muguruma Tomoaki

  Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University

• Sakanaka Kazunobu

  Advanced Technology Laboratory Kubota Corporation

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