Purification and Properties of Cytosine Deaminase from Aspergillus fumigatus
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概要
- 論文の詳細を見る
Cytosine deaminase (EC 3.5.4.1) from Aspergillus fumigatus IFO 5840,which is the first cytosine deaminase to be found in a mould, was purified 150-fold with an overall yield of 0.75%, to homogeneity judging from disc and SDS-polyacrylamide gel electrophoresis. The enzyme was a monomer of 32 kDa. Besides cytosine, the enzyme stoichiometrically deaminated 5-methylcytosine and 5-fluorocytosine : the activity toward them was 100 : 28 : 43,and the apparent K_m values for them were 2,36,and 6.5 mM, respectively. The enzyme had a pH optimum at around pH 7 and temperature optimum at 35℃. The enzyme activity was inhibited by heavy metal ion such as Fe^<2+>, Cu^<2+>, Hg^<2+>, and Pb^<2+> at 0.1 mM, and by p-chloromercuribenzoate, o-phenanthroline, ATP, and UTP at 1 mM.
- 社団法人日本生物工学会の論文
- 1991-10-25
著者
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Yagyu Takehiko
Department Of Microbiology College Of Natural Science Keimyung University
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YU TAE-SHICK
Department of Microbiology, College of Natural Science, Keimyung University
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KIM JAE-KUEN
Department of Microbiology, College of Natural Science, Keimyung University
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KATSURAGI TOHORU
Fermentation Chemistry Laboratory, College of Agriculture, University of Osaka Prefecture
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SAKAI TAKUO
Fermentation Chemistry Laboratory, College of Agriculture, University of Osaka Prefecture
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TONOMURA KENZO
Fermentation Chemistry Laboratory, College of Agriculture, University of Osaka Prefecture
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Kim Jae-kuen
Department Of Microbiology College Of Natural Science Keimyung University
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