Purification and Characterization of Acid-stable Protopectinase Produced by Aspergillus awamori in Solid-state Fermentation
スポンサーリンク
概要
- 論文の詳細を見る
Aspergillus awamori IFO 4003 produced an acid-stable protopectinase in solid-state fermentation using wheat bran as the medium. The enzyme was purified to a homogeneous preparation with anion-exchange, hydrophobic, and size-exclusion chromatography. The enzyme was a monomeric protein of 52 kDa, by SDS-PAGE analysis, with an isoelectric point of pH 3.7. The optimum pH for enzyme activity was 2.0,and it was most active at 50℃ (at pH 2.0) and was stable up to 50℃(at pH 2.0). The enzyme showed pectin-releasing activity toward protopectins from various origins, especially on lemon protopectin. An outstanding characteristic of the enzyme was its extreme stability in acidic conditions : the enzyme activity was not lost after incubating at pH 2.0 and 37℃ for 24 h.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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OZAWA Akira
University of Tsukuba
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Ozawa Akira
Department Of Dermatology Division Of Specialized Clinical Science Tokai University School Of Medici
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Ozawa Akira
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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NAGAI Masaru
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Nagai M
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Nagai Masaru
Department Of Electrical And Electronic Engineering Muroran Institute Of Technology
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Ozawa Akira
The Institute Of Physical And Chemical Research (riken)
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Ozawa Akira
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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