Cloning and Characterization of Zymomonas mobilis Genes Encoding Extracellular Levansucrase and Invertase
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概要
- 論文の詳細を見る
The genes encoding the extracellular levansucrase and invertase of Zymomonas mobilis have been cloned and sequenced. The levansucrase gene, sucZE2, spans 1269 bp and encodes an M_r 46,790 polypeptide, and the invertase gene, sucZE3, is of 1239 bp and encodes an M_r 46,110 polypeptide. The 5'-terminal sequences of both genes corresponded to the N-terminal amino acid sequences of the secreted levansucrase and invertase, implying that the secretion of both enzymes does not involve proteolytic processing of the N-terminals. Both enzyme molecules appear to carry no typical N-terminal secretion signal. Significant homology between sucZE2 and sucZE3 was observed, but both genes showed no homology to the gene encoding an intracellular invertase coexisting in Z. mobilis. Two genes, sucZE2 and sucZE3, are possibly placed in an operon because the expression of two genes were simultaneously controlled by the regulator gene zliE, previously identified.
- 社団法人日本農芸化学会の論文
- 1995-02-23
著者
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YANASE Hideshi
Department of Biotechnology, Faculty of Engineering, Tottori University
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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KAWASAKI Haruhiko
Department of Agricultural Chemistry, University of Osaka Prefecture
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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TONOMURA KENZO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture University Of Osaka Prefecture
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Kawasaki H
Department Of Environmental Materials Science Tokyo Denki University
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kyono Kiyoshi
Department of Applied Biochemistry, Faculty of Agriculture, University of Osaka Prefecture
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Kyono Kiyoshi
Medicinal Chemistry Research Laboratories Tanabe Seiyaku Co. Ltd.
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