Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori

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概要

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• An extracellular endo-polygalacturonase (PGase) produced by Aspergillus awamori IFO 4033 was isolated from the culture filtrate. The enzyme was purified to a homogeneous preparation with cation-exchange and size-exclusion chromatographies. Its properties were investigated, comparing them with that of recombinant pgx2 gene product, a PGase having protopectinase activity. This enzyme was a monomeric protein of 41 kDa, with an isoelectric point of pH 6.1. The characteristics of this PGase substantially coincide, with that of recombinant pgx2 gene product, and the PGase is assumed to be native pgx2 gene product. The production of PGase-X2 was confirmed to be regulated by ambient pHs.

• 社団法人日本農芸化学会の論文
• 2000-08-23

著者

• Yoshikawa K

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• Yoshikawa Kentaro

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• TERASHITA Takao

  Department of Food and Nutrition, Faculty of Agriculture, Kinki University

• SAKAI TAKUO

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Yoshikawa K

  Department Of Bioscience Faculty Of Bioresources Mie University

• NAGAI Masaru

  Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University

• KATSURAGI Tohoru

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Nagai M

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• Nagai Masaru

  Department Of Electrical And Electronic Engineering Muroran Institute Of Technology

• YOSHIKAWA Kentaro

  Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University

• Sakai Takuo

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• Sakai Takuo

  Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture

• Sakai Takuo

  Center For Research And Development Of Bioresources University Of Osaka Prefecture

• Sakai Takuo

  Department Of Food Science Faculty Of Agriculture Kinki University

• Sakai Takuo

  Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture

• Sakai Takuo

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity

• Sakai Takuo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Katsuragi Tohoru

  Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture

• Katsuragi Tohoru

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Katsuragi Tohoru

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)

• Terashita T

  Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University

• Terashita T

  Kinki Univ. Nara Jpn

• Terashita Takao

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Yoshikawa Kentaro

  Department Of Food And Nutrition Faculty Of Agriculture Kinki University

• Yoshida Kouji

  Department Of Apple Research National Institute Of Fruit Tree Science

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