Glycolaldehyde-Forming Route in Bacillus subtilis in Relation to Vitamin B_6 Biosynthesis
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概要
- 論文の詳細を見る
Glycolaldehyde (GA) was shown to be a precursor of vitamin B_6 (B_6), and to be formed from glycolate by glycolaldehyde dehydrogenase (GADH) in Escherichia coli. In this study, we show the glycolaldehyde-forming route in B_6 biosynthesis in Bacillus subtilis. In the crude extract of B.subtilis, the oxidizing activity of GADH was detected. However, coexisting NADH/NADPH oxidase activity interfered with the determination of the reducing (GA-forming) activity of GADH. NADH/NADPH oxidase was purified and identified as the product of ahpF. In an ahpF disruptant, NADH/NADPH activity was almost eliminated, but the reducing activity of GADH was not detected. We also investigated another possible GA-forming enzyme, glyoxal reductase (GR). GR was partially purified and identified as the product of yvgN. yvgN disruptant did not require B_6, and retained the ability to synthesize the same amount of B_6 as the wild-type strain. From these results, we concluded that neither GADH nor GR is involved in B_6 biosynthesis in B.subtilis.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Sakai Akihiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Tani Yoshiki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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KATAYAMA KEISI
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Tani Yoshiki
Graduate School Of Biological Sciences Advanced Institute Of Science And Technology
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Katayama Keisi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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