Cytosine Deaminase from Escherichia coli : Production, Purification, and Some Characteristics(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
We wanted to obtain a large amount of cytosine deaminase from Escherichia coli. This enzyme deaminates 5-fluorocytosine and is thermostable. It is needed for trial use in a novel chemotherapy for cancer in combination with 5-fluorocytosine. We improved the conditions of its production by examining the culture conditions. A pH-stat culture with the culture pH controlled at around 8.5 using citric acid gave efficient production of cell mass and enzyme activity. The enzyme was purified 1200-fold from the cell-free extract, to homogeneity, using two kinds of affinity adsorbents prepared for this purpose. The enzyme had a molecular weight of 200,000 (gel permeation), and seemed to be an SH-enzyme. The purified enzyme was thermostable.
- 社団法人日本農芸化学会の論文
- 1986-07-23
著者
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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SAKAI Takuo
Laboratory of Food Microbiological Engineering, Faculty of Agriculture Kinki University
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Laboratory Of Cell Biotechnology Graduate School Of Biological Sciences Nara Institute Of Science An
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Matsumoto K
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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TONOMURA Kenzo
Laboratory of Fermentation Chemistry, College of Agriculture, University of Osaka Prefecture
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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