Liberation of Sucrose-Hydrolyzing Enzymes from Cells by the zliS Gene Product That Mediates Protein Secretion in Zymomonas mobilis

概要

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A 1.7kb DNA fragment containing both the zliE and zliS genes complements the sucrose-deficiency of the mutant, Z6C-S2,derived from Zymomonas mobilis ATCC 29191,and the mutant containing both genes produces sucrose-hydrolyzing enzymes extracellularly. The mutant containing zliE accumulates the enzymes within the cells, while that containing zliS does not produce the enzymes(Kondo, T. et al., Biosci. Biotech. Biochem., 58,526-530,1994). In the mutant cells containing zliE, most of the sucrose-hydrolyzing enzymes were bound on the cell sufrace, and these enzymes were liberated when incubated with either washing, suspension or extract of the cells containing zilS. These results suggest that a product of the zliS gene effects the cell membrane to stimulate the liberation of cell-associated proteins.
公益社団法人日本生物工学会の論文
1994-04-25