Cloning and Characterization of a Pair of Genes That Stimulate the Production and Secretion of Zymomonas mobilis Extracellular Levansucrase and Invertase
スポンサーリンク
概要
- 論文の詳細を見る
A 1. 7-kb DNA fragment cloned from Zymomonas mobilis genomic DNA complemented the inability to grow on sucrose of a Suc^- mutant of Z. mobilis that was deficient in the production of both extracellular levansucrase and invertase. Analysis of the nucleotide sequence of the fragment found two open reading frames (ORFs), both of which did not correspond to the structural gene for the levansucrase or the invertase. By subcloning each ORF into two different Suc^- mutants of Z. mobilis, it has been found that the first ORF (gene zliE) activates the production of the extracellular levansucrase and invertase, and the second ORF (gene zliS) stimulates the secretion of the two enzymes. Gene zliS might contribute to the secretion of proteins having no signal peptide. The expression of zliE and zliS seemed to be under the control of the same promoter.
- 社団法人日本農芸化学会の論文
- 1994-03-23
著者
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Kondo Yasushi
Department of Physics, Kinki University
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YANASE Hideshi
Department of Biotechnology, Faculty of Engineering, Tottori University
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Toyoda A
Envysis Co. Ltd. Shikama Jpn
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Toyoda Atsushi
Department Of Bioengineering Faculty Of Engineering Soka University
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Toyoda Atsushi
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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KAWASAKI Haruhiko
Department of Agricultural Chemistry, University of Osaka Prefecture
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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TONOMURA KENZO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture University Of Osaka Prefecture
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Kondo Yasushi
Department Of Physics Kinki University
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Kawasaki H
Department Of Environmental Materials Science Tokyo Denki University
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Kondo Y
Ensuiko Sugar Refining Co. Ltd. Yokohama Jpn
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Fukushi Hideaki
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
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Fukushi Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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