Fermentation of Lactose by Zymomonas mobilis Carrying a Lac^+ Recombinant Plasmid
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概要
- 論文の詳細を見る
Lac^+ recombinant plasmids encoding a β-galactosidase fused protein and lactose permease of Escherichia coli were introduced into Zymomonas mobilis. The fused protein was expressed with 450 to 5,860 Miller units of β-galactosidase activity, and functioned as lactase. Raffinose uptake by Z. mobilis CP4 was enhanced in the plasmid-carrying strain over the plasmid-free strain, suggesting that the lactose permease was functioning in the organism. Z. mobilis carrying the plasmid could produce ethanol from lactose and whey, but could not grow on lactose as the sole carbon source. It was found that the growth of the organism was inhibited by either galactose or the galactose liberated from lactose.
- 社団法人日本生物工学会の論文
- 1988-08-25
著者
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YANASE Hideshi
Department of Biotechnology, Faculty of Engineering, Tottori University
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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TONOMURA KENZO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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KURII JUNN
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Kurii J
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kurii Junn
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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