Plasmid-determined Dehalogenation of Haloacetates in Moraxella Species
スポンサーリンク
概要
- 論文の詳細を見る
A strain of Moraxella sp. capable of assimilating fluoroacetate (FA) as the sole source of carbon was isolated. It was resistant to mercuric chloride and antibiotics, such as penicillin G and kanamycin. Mercury reductase and haloacetate halidohydrolase were found in the cell-free extract of the organism. Two kinds of halidohydrolase were found on purification by DEAE-cellulose column chromatography; one was active for FA and chloroacetate (CA) (halidohydrolase 1), and the other was active for CA but not FA (halidohydrolase 2). Two types of cured strain were obtained by mitomycin C; type I lost halidohydrolase 2, whereas type II became sensitive to mercuric chloride and lost halidohydrolase 1 and 2. On electrophoresis of plasmid on agarose gel, a plasmid band was observed in a wild strain and a shorter band in type I. However, no band was observed in type II. Transferring of the plasmid by conjugation from a wild cell to a type II cell gave an exconjugant which possessed halidohydrolase 1 and 2 and became resistant to mercury. These results indicate that both halidohydrolase 1 and 2 and also mercury reductase are determined by the plasmid in the organism.
- 社団法人 日本農芸化学会の論文
著者
-
Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Tone Noriko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
関連論文
- Catalysis-Linked Inactivation of Fluoroacetate Dehalogenase by Ammonia: A Novel Approach to Probe the Active-Site Environment
- FM-P46 Optimization of bacteriocin production by thermotolerant lactic acid bacteria, Pediococcus KKU 170, isolated from Thai local fermented food(Section VIII Fermentation and Microbial Technology)
- Production of Monoclonal Antibody against Protopectinase from Kluyveromyces wickerhamii : Studies of Enzymes Produced by Yeasts (IX).
- Purification and Properties of Extracellular β-Mannanases Produced by Enterococcus casseliflavus FL2121 Isolated from Decayed Konjac
- Fermentation of Lactose by Zymomonas mobilis Carrying a Lac^+ Recombinant Plasmid
- Cloning and Heterologous Expression of Gene Encoding A Polygalacturonase from Aspergillus awamori
- Purification and Characterization of an Endo-Polygalacturonase from a Mutant of Saccharomyces cerevisiae
- Isolation and Genetic Determination of a Saccharomyces cerevisiae Mutant That Produces an Endo-Polygalacturonase
- Molecular Cloning and Expression of a Polygalacturonase Gene in Saccharomyces cerevisiae
- Expression of the Extracellular Levansucrase and Invertase Genes from Zymomonas mobilis in Escherichia coli Cells
- Purification and Characterization of α-Galactosidase from the Yeast Trichosporon cutaneum JCM 2947
- Microbial Degradation of Poly(3-Hydroxybutyrate) and Polycaprolacutone by Filamentous Fungi
- Cloning and Expression in Escherichia coli of Sucrose Phosphorylase Gene from Leuconostoc mesenteroides No. 165
- Screening for Bacteria Producing Sucrose Phosphorylase and Characterization of the Enzymes
- Cloning and Characterization of Zymomonas mobilis Genes Encoding Extracellular Levansucrase and Invertase
- Cloning and Characterization of a Pair of Genes That Stimulate the Production and Secretion of Zymomonas mobilis Extracellular Levansucrase and Invertase
- Plasmid pUO2 Determining Haloacetate Dehalogenase and Mercury Resistance in Pseudomonas sp.
- Expression of Aspergillus oryzae Phytase Gene in Aspergillus oryzae RIB40 niaD^-(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purificatioin, Crystallization, and Characterization of the Extracellular Invertase from Zymomonas mobilis
- Purification, Crystallization, and Properties of the Extracellular Levansucrase from Zymomonas mobilis
- Purification and Characterization ofα-Glucosidase from Torulaspora pretoriensis YK-1
- Cloning, Sequencing, and Characterization of the Intracellular Invertase Gene from Zymomonas mobilis(Microbiology & Fermentation Industry)
- DELETION AND RECOMBINATION OF PLASMID pU01 CODING FOR DEHALOGENATION OF HALOACETATE AND MERCURY RESISTANCE
- PLASMID ENCODING MERCURY REDUCTASE AND HALOACETATE HALIDOHYDROLASE IN DRUG-RESISTANT BACTERIA
- Transformation of Zymomonas mobilis with Plasmid DNA(Microbiology & Fermentation Industry)
- Construction of a Promoter-cloning Vector in Zymomonas mobilis
- Liberation of Sucrose-Hydrolyzing Enzymes from Cells by the zliS Gene Product That Mediates Protein Secretion in Zymomonas mobilis
- Construction of a New Shuttle Vector for Zymomonas mobilis
- Microbial Metabolism of Nitriles in Pseudomonas sp. : Metabolism of Nitriles in Microorganisms. (VII)
- DEHALOGENATION OF HALOACETATE BY PLASMID-BEARING DRUG-RESISTANT STRAIN OF MORAXELLA SP.
- Cloning and Sequence Analysis of a Plasmid-encoded 2-Haloacid Dehalogenase Gene from Pseudomonas putida No. 109
- Selection of a Novel Baking Strain from the Torulaspora Yeasts
- Purification and Characterization of Invertase from Torulaspora pretoriensis YK-1
- Formation of β-Cyanoalanine by Cyanide-resistant Strain Enterobacter sp. 10-1
- Purification and Crystallization of a β-Cyanoalanineforming Enzyme from Enterobacter sp. 10-1
- Shuttle Vectors for Zymomonas mobilis
- Cloning and Expression in Escherichia coli of the Haloacetate Dehalogenase Genes from Moraxella Plasmid pUO1
- Cleavage Mapsof Dehalogenation Plasmid pUOl and Its Deletion Derivative Harbored in Moraxella sp.
- Molecular Modification of a Dehalogenation Plasmid Originated from a Moraxella Species in a Foreign Host
- Some Properties of a β-Cyanoalanine-forming Enzyme of Enterobacter sp. 10-1
- Purification, Crystallization and Some Properties of β-Cyano-L-alanine-degrading Enzyme in Pseudomonas sp. 13
- Possible Role of Cell Wall Mannan in the Secretion of the Protopectin-solubilizing Enzyme of Trichosporon penicillatum SNO-3
- Metabolism of Thiophene-2-carboxylate by a Photosynthetic Bacterium
- Microbial Utilization of β-Cyano-L-alanine by Pseudomonas sp. 13
- A Cointegrate Plasmid Constructed in vivo between a Plasmid that Mediates Raffinose Catabolism in Escherichia coli and an IncP-1 Plasmid, R68.45
- Fusion of Spheroplasts and Genetic Recombination of Zymomonas mobilis
- Cloning of a raf Operon That Mediates Raffinose Catabolism in Escherichia coli
- Purification, Crystallization and Properties of Haloacetate Halidohydrolase from Pseudomonas Species
- Plasmid-determined Dehalogenation of Haloacetates in Moraxella Species
- Purification and Properties of Haloacetate Halidohydrolase Specified by Plasmid from Moraxella sp. Strain B
- Catalysis-Linked Inactivation of Fluoroacetate Dehalogenase by Ammonia: A Novel Approach to Probe the Active-Site Environment.