Microbial Degradation of Poly(3-Hydroxybutyrate) and Polycaprolacutone by Filamentous Fungi
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概要
- 論文の詳細を見る
Five strains were isolated from soil as fungi able to degrade both poly(3-hydroxybutyrate) (PHB) and polycaprolactone (PCL), and one of the strains, D218,identified as Paecilomyces lilacinus, was selected. In 10 d, D218 degraded PHB almost completely and 10% of the PCL, each at 0.1% in the culture medoa. Strain D218 excreted PHB and PCL depolymerases in media containing either PHB, PCL or PHB plus PCL. Both depolymerase activities were reduced when the medium was supplemented with either soluble starch, lactose or glucose. The optimum conditions for PHB depolymerase were pH 6.5 to 7.5 at 50℃, while those for PCL depolymerase were pH 3.5 to 4.5 at 30℃. In the reaction mixtures used for the enzyme assays, the formation of 3-hydroxybutyrate from PHB and ε-caprolactone from PCL was confirmed by high performance liquid chromatography.
- 社団法人日本生物工学会の論文
- 1995-09-25
著者
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ODA Yuji
Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro
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TONOMURA KENZO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Urakami Teizi
Mitsubishi Gas Chemical Company
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ASARI HIROYUKI
Department of Food Science and Technology, Fukuyama University
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Asari Hiroyuki
Department Of Food Science And Technology Fukuyama University
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Oda Yuji
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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