Fusion of Spheroplasts and Genetic Recombination of Zymomonas mobilis
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概要
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Spheroplasts of Zymomonas mobilis (Z-6) were prepared by cultivating it in a hypertonic medium containing penicillin G or glycine at 30°C for more than 6 hr. The reversion of Spheroplasts to a bacillary form was observed at frequencies of 10-2 to 10-3 when Spheroplasts were incubated on hypertonic solid agar plates overlaid with soft agar. Spheroplast fusion was attempted with polyethylene glycol 6000 by crossing two mutants lacking the ability to ferment sugars. Fusants were selected, and obtained at high frequencies. The properties of a stable fusant are discussed.
- 社団法人 日本農芸化学会の論文
著者
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Yasui Masaru
Department Of Applied Physics Faculty Of Engineering Nagoya University:(present Address) Faculty Of
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Miyazaki Takayuki
Department Of Electronic Engineering Faculty Of Engineering Gunma University
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YANASE Hideshi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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YASUI Masaru
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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YASUI Masaru
Department of Agricultural Chemistry, College of Agriculture, The University of Osaka Prefecture
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