Cloning and Sequence Analysis of a Plasmid-encoded 2-Haloacid Dehalogenase Gene from Pseudomonas putida No. 109
スポンサーリンク
概要
- 論文の詳細を見る
The 2-haloacid dehalogenase of Pseudomonas putida No. 109 was mediated by a 74-kb conjugative plasmid, which was transferred by mating into Pseudomonas and Escherichia coli and there expressed the dehalogenase. A 2.8-kb EcoRI-fragment generated from the plasmid was cloned and sequenced. The dehalogenase gene (dehH109) was identified by comparison with the N-terminal amino acid sequence and the molecular weight of the enzyme protein. The gene dehH109 coded for a 224-amino acid protein of M_r 25,231, which showed significant homology to the other four L-specific 2-haloacid dehalogenases from Pseudomonas sp. CBS3, P. putida AJ1, and Xanthobacter autotrophicus GJ10 and also to the haloacetate dehalogenase H-2 from Moraxella sp. strain B, but no homology with another haloacetate dehalogenase H-1 and the D-specific 2-haloacid dehalogenase from P. putida AJ1.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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Maeda Takuya
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Maeda T
Kobe Univ. Kobe Jpn
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Toyama Tohru
Department of Agricultural Chemistry, University of Osaka Prefecture
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Nishino Humiko
Department of Agricultural Chemistry, University of Osaka Prefecture
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Toyama Tohru
Department Of Agricultural Chemistry University Of Osaka Prefecture
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Nishino Humiko
Department Of Agricultural Chemistry University Of Osaka Prefecture
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Tonomura Kenzo
Department Of Agricultural Chemistry University Of Osaka Prefecture
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Maeda Takuya
Department of Agricultural Chemistry, University of Osaka Prefecture
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