Cloning and Heterologous Expression of Gene Encoding A Polygalacturonase from Aspergillus awamori
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概要
- 論文の詳細を見る
A polygalacturonase gene of Aspergillus awamori IFO 4033 was cloned by genomic Southern hybridization with a probe of a DNA fragment synthesized by PCR. This was done using primers constructed based on the N-terminal amino acid sequence of a polygalacturonase, protopectinase-AS, produced by the strain and the consensus internal amino acid sequence of fungal polygalacturonases. The cloned polygalacturonase gene, containing an ORF, encodes 362 amino acids, including a 52-bp intron. It contains the consensus nucleotide sequence of PacC binding sites, and its expression was appeared to be regulated by ambient pH. After the intron was excised, the cloned gene was inserted into an expression plasmid for yeast, pMA91,and introduced into Saccharomyces cerevisiae to be expressed. The expressed gene product was purified to a homogeneous preparation, and this confirmed that the polygalacturonase produced was the product of the cloned gene.
- 社団法人日本農芸化学会の論文
- 2000-08-23
著者
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OZAWA Akira
University of Tsukuba
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Ozawa Akira
Department Of Dermatology Division Of Specialized Clinical Science Tokai University School Of Medici
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KAWASAKI Haruhiko
Department of Agricultural Chemistry, University of Osaka Prefecture
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SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Ozawa Akira
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University
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NAGAI Masaru
Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University
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KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Nagai M
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Nagai Masaru
Department Of Electrical And Electronic Engineering Muroran Institute Of Technology
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Sakai Takuo
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Sakai Takuo
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Center For Research And Development Of Bioresources University Of Osaka Prefecture
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Sakai Takuo
Department Of Food Science Faculty Of Agriculture Kinki University
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Sakai Takuo
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Sakai Takuo
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Kawasaki Haruhiko
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Ozawa Akira
The Institute Of Physical And Chemical Research (riken)
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Kawasaki Haruhiko
Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University
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Ozawa Akira
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
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