Optimal Conditions for Production of(R)-1-Phenylpropanol by Fusarium moniliforme Strain MS31
スポンサーリンク
概要
- 論文の詳細を見る
Resting cells of Fusarium moniliforme strain MS31 produced(R)-1-phenylpropanol from propylbenzene. The components of the medium and the reaction conditions were adjusted to increase the specific activity of the hydroxylating enzyme involved. Glucose and sodium nitrate were selected as carbon and nitrogen sources, respectively. The substrate, propylbenzene, inhibited fungal growth and the activity of the enzyme. Acetoin added to the medium increased both growth and activity of the enzyme, and hydroxylation of propylbenzene increased by 1.4-fold. Maximum bioconversion of propylbenzene by resting cells of the fungus was at 25-30℃ and pH 7.0 with cells at concentration of 40mg(dry)per milliliter of reaction mixture. Conversion was accelerated as soon as propylbenzene was added; slowing 2h later. In the end, F.moniliforme strain MS31 produced(R)-1-phenylpropanol with an enantiomeric excess of 98% at the concentration of 16mM(2.2mg・ml^<-1>.
- 社団法人日本生物工学会の論文
- 2001-09-25
著者
-
Tani Yoshiki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
KATSURAGI Tohoru
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Katsuragi Tohoru
Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture
-
Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Katsuragi Tohoru
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
-
Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
-
Tani Yoshiki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
UZURA ATSUKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Uzura Atsuko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Tani Yoshiki
Graduate School Of Biological Sciences Advanced Institute Of Science And Technology
関連論文
- Purification, Characterization, and Gene Cloning of Glycerol Dehydrogenase from Hansenula ofunaensis, and Its Expression for Production of Optically Active Diol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Properties of Cephalosporin-C Deacetylase from the Yeast, Rhodotorula glutinis 38B1, Useful for Bioconversion of 7-Aminocephalosporanic Acid Derivatives
- Ester Synthesis by NAD^+ -dependent Dehydrogenation of Hemiacetal : Production of Methyl Formate by Cells of Methylotrophic Yeasts
- A Non-Conventional Dissimilation Pathway for Long Chain n-Alkanes in Acinetobacter sp. M-1 That Starts with a Dioxygenase Reaction
- Production of Fructosyl Lysine Oxidase from Fusarium oxysporum S-1F4 on Autoclave-browned Medium
- Purification and Properties of FructosylLysine Oxidase from Fusarium oxysporum S-1F4
- Use of Long-chain n-Alkanes (C_13-C_44) by an Isolate, Acinetobacter sp. M-1
- Purification and Properties of Cytosine Deaminase from Aspergillus fumigatus
- Production of Monoclonal Antibody against Protopectinase from Kluyveromyces wickerhamii : Studies of Enzymes Produced by Yeasts (IX).
- Effects of Organic Solvents and Chemical Modification on the Activity of Glycerol Dehydrogenase(Microbiology & Fermentation Industry)
- Characterization of Nitrile Hydratase and Amidase, Which Are Responsible for the Conversion of Dinitriles to Mononitriles, from Corynebacterium sp.(Microbiology & Fermentation Industry)
- Bioconversion of Dinitrile to Mononitrile, a Tranexamic Acid Intermediate, by Corynebacterium sp.(Microbiology & Fermentation Industry)
- 416 Methyl formate production by methanol-utilizing yeast, Pichia methanolica (P. aganobii)
- Cloning and Heterologous Expression of Gene Encoding A Polygalacturonase from Aspergillus awamori
- Purification and Characterization of Acid-stable Protopectinase Produced by Aspergillus awamori in Solid-state Fermentation
- Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
- Investigation of 1-Deoxy-D-Xylulose 5-Phosphate Synthase and Transketolase of Bacillus subtilis in Relation to Vitamin B_6 Biosynthesis
- Characterization of a Flavinogenic Mutant of Methanol Yeast Candida boidinii and Its Extracellular Secretion of Riboflavin
- Stabilization of Cytosine Deaminase from Bakers' Yeast by Immobilization(Microbiology & Fermentation Industry)
- Purification and Some Properties of Cytosine Deaminase from Bakers' Yeast(Microbiology & Fermentation Industry)
- Cytosine Deaminase from Escherichia coli : Production, Purification, and Some Characteristics(Microbiology & Fermentation Industry)
- Affinity Chromatography of Cytosine Deaminase from Escherichia coli with Immobilized Pyrimidine Compounds(Microbiology & Fermentation Industry)
- Visual Assay for Specific Inhibitors of Adenosine Deaminase with Agar Plates Containing pH Indicator
- Polyol Production by Culture of Methanol-Utilizing Yeast
- Functional Analysis of Genes Encoding Putative Oxidoreductases in Aspergillus oryzae, Which Are Similar to Fungal Fructosyl-Amino Acid Oxidase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Production of Raw Cassave Starch-Digestive Glusoamylase by Rhizopus sp. in Liquid Culture
- Bacterial Communities in Petroleum Oil in Stockpiles (Environmental Biotechnology)
- Occurrence of Fructosyl-Amino Acid Oxidase-Reactive Compounds in Fungal Cells
- Characterization of Two Fructosyl-Amino Acid Oxidase Homologs of Schizosaccharomyces pombe (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Recent Progress of Vitamin B_6 Biosynthesis
- Mechanism for Degradation of Poly(Sodium Acrylate) by Bacterial Consortium No. L7-98
- serC Is Involved in Vitamin B_6 Biosynthesis in Escherichia coli but not in Bacillus subtilis
- yaaD and yaaE Are Involved in Vitamin B_6 Biosynthesis in Bacillus subtilis
- Involvement of Cytochrome P450 in Hydroxylation of Propylbenzene by Fusarium moniliforme Strain MS31
- Conversion of Various Aromatic Compounds by Resting Cells of Fusarium moniliforme Strain MS31
- Optimal Conditions for Production of(R)-1-Phenylpropanol by Fusarium moniliforme Strain MS31
- Stereoselective Oxidation of Alkylbenzenes by Fungi
- Glycolaldehyde-Forming Route in Bacillus subtilis in Relation to Vitamin B_6 Biosynthesis
- Selective Oxidation of 2-Methylnaphthalene to 2-Methyl-1-naphthol by Rhodococcus sp. M192
- Purification and Molecular Cloning of an Intracellular 3-Hydroxybutyrate-Oligomer Hydrolase from Paucimonas lemoignei(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Soluble Cytochromes c of a KCN-resistant Mutant of an Obligate Methylotroph, Methylomonas sp.(Microbiology & Fermentation Industry)
- Purification and Characterization of Alcohol Dehydrogenase Reducing N-Benzyl-3-Pyrrolidinone from Geotrichum capitatum(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Screening of Carbon Dioxide-Requiring Extreme Oligotrophs from Soil
- Production of Raw Cassava Starch-Digestive Glucoamylase by a 2-Deoxyglucose-Resistant Mutant of Rhizopus sp.
- Microbial Conversion of 2-Methylnaphthalene to 2-Methyl-1-naphthol and Menadione
- Enzymatic Alteration in the Shikimate Pathway during Derivation of Menaquinone-4-producing Mutants of Flavobactevium sp. 238-7(Microbiology & Fermentation Industry)
- Menaquinone-4 Production by a Sulfonamide-resistant Mutant of Flavobacterium sp. 238-7(Microbiology & Fermentation Industry)
- Derivation of a Glycerol-negative Mutant of a Methylotrophic Yeast, Candida boidinii, and Accumulation of Glycerol in Methanol Medium(Microbiology & Fermentation Industry)
- Purification and Characterization of an Enzyme That Has Dihydroxyacetone-Reducing Activity from Methanol-Grown Hansenula ofunaensis
- Production of Optically Active 1,2,4-Butanetriol from Corresponding Racemate by Microbial Stereoinversion(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Enlarged and Astaxanthin-Accumulating Cyst Cells of the Green Alga Haematococcus pluvialis
- Degradation of Dibenzothiophene by Sulfate-Reducing Bacteria Cultured in the Presence of Only Nitrogen Gas
- Microbial Degradation of Poly (sodium acrylate)
- Selective Release of Menaquinone-4 from Cells of a Mutant of Flavobacterium sp. 238-7with a Detergent
- Vitamin K_2 (Menaquinone) : Screening of Producing Microorganisms and Production by Flavobacterium meningosepticum
- Improvement of ATP Production with Cells of a Methylotrophic Yeast, Candida boidinii, by Genetic Engineering.