Mechanism for Degradation of Poly(Sodium Acrylate) by Bacterial Consortium No. L7-98

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概要

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• Poly (sodium acrylate) (PSA) can be degraded by consortia of several bacterial species. We investigated the degradation mechanism for PSA (average molecular weight, 2100) by consortium no. L7-98, PSA was used as the sole carbon source in a mineral salt medium. After cultivation, the PSA had a range of molecular weights, including low-molecular-weight compounds, which were purified by gel-permeation and reversed-phase column chromatography. One purified compound, B1, with the molecular weight of 200, had a carbonyl group next to the terminus, according to ^1H and ^<13>C nuclear magnetic resonance spectrometry and X-ray analysis of the crystal structure. Two categories of metabolites of PSA were detected in the culture by electrospray ionization mass spectrometry. Results of high-resolution mass spectrometry (HR-MS) suggested that one kind of compounds had a carbonyl group and that the other kind of compounds had an aldehyde group and a double bond. Compounds having the molecular weights of 200 and 272 were rapidly produced from an acrylic acid oligomer with the molecular weight of 258 by resting cells of the consortium. HR-MS showed that a methylene group at the terminal unit was oxidized to a carbonyl group and that the compound with the molecular weight of 200 was compound B1. From these results, we propose that the degradation pathway of PSA involves (i) oxidation of a methylene group to a carbonyl group next to the terminus, (ii) decarboxylation to form an aldehyde group and dehydrogenation to form a double bond between the terminal unit and the next unit, and (iii) oxidation of the aldehyde group to a carboxyl group followed by elimination of an acetic acid.

• 社団法人日本生物工学会の論文
• 2003-05-25

著者

• Tani Yoshiki

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• KATSURAGI Tohoru

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• TSUTSUMI Ken

  Graduate School of Material Science, Nara Institute of Science and Technology

• KAKIUCHI Kiyomi

  Graduate School of Material Science, Nara Institute of Science and Technology

• Katsuragi Tohoru

  Fermentation Chemistry Laboratory College Of Agriculture University Of Osaka Prefecture

• Katsuragi Tohoru

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Katsuragi Tohoru

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)

• Tsutsumi Ken

  Graduate School Of Life And Environmental Sciences University Of Tsukuba:sugadaira Motane Research C

• Tsutsumi Ken

  Graduate School Of Materials Science Nara Institute Of Science And Technology (naist)

• Tani Y

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci

• Tani Yoshiki

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Kakiuchi Kiyomi

  Graduate School Of Materials Science Nara Institute Of Science And Technology (naist)

• Kakiuchi Kiyomi

  Department Of Applied Fine Chemistry Faculty Of Engineering Osaka University

• IWAHASHI MASAHIDE

  Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)

• Tani Yoshiki

  Graduate School Of Biological Sciences Advanced Institute Of Science And Technology

• Iwahashi Masahide

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)

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