serC Is Involved in Vitamin B_6 Biosynthesis in Escherichia coli but not in Bacillus subtilis

概要

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The possible involvement of serC in vitamin B_6 (B_6) biosynthesis in Bacillus subtilis was investigated and compared with that in Escherichia coli. The genes of E. coli and B. subtilis were disrupted with pBEN66 and pMutinl-derived integration vectors, respectively. Nutrient requirement analyses showed that the serC-disrupted E. coli mutant required pyridoxine (PN) and L-serine, and lacked the ability to synthesize B_6. Glycolaldehyde (GA), a confirmed precursor of B_6, could replace PN and support the growth of the disruptant. However, the serC-disrupted E. coli mutant grown in a minimal medium supplemented with L-serine and GA synthesized B_6 at a level less than 20% of that synthesized by the wild type. In contrast to E. coli, the serC-disrupted B. subtilis mutant required L-serine or glycine for growth, but did not require PN. The serC disruptant retained its ability for B_6 biosynthesis and produced almost the same amount of PN as the wild type. GA had no effect on the growth and level of B_6 biosynthesis of both the wild type and the serC disruptant. These results lead to the conclusion that serC is directly involved in B_6 biosynthesis in E. coli, but not in B. subtilis.
社団法人日本生物工学会の論文
2002-03-25