Soluble Cytochromes c of a KCN-resistant Mutant of an Obligate Methylotroph, Methylomonas sp.(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A KCN-resistant mutant, Methylomonas sp. YK 56, contained three kinds of soluble cytochromes c (cytochromes c-I, c-II, and c-III) though the wild type strain contained two kinds (cytochromes c-I and c-III). The proportion of the three cytochromes c of the mutant were 2.4, 71.5, and 26.1%, and that of the two cytochromes c of the wild type strain were 2.1 and 97.9%, respectively. Cytochromes c-II and c-III of the mutant were purified by a procedure involving ammonium sulfate fractionation and DEAE-, CM-cellulose, and Sephadex G-150 column chromatography. Cytochrome c-II was obtained as crystals with ammonium sulfate. Both absorption peaks of the α-band of the two cytochromes c were at 551.5nm at room temperature and the β-band of cytochrome c-II had a shoulder at 530nm. Molecular weights of the two cytochromes c were 16,000 and 20,000, respectively and their isoelectric points were 4.1 and 3.5, respectively.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
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Yoon B
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Yamada H
Nagoya Univ. Nagoya Jpn
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Yoon Byung
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Tani Y
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:department Of Biosci
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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