Extracellular Production of Menaquinone-4 by a Mutant of Flavobacterium sp. 238-7 with a Detergent-Supplemented Culture

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概要

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• Culture conditions for the extracellular production of menaquinone (MK) by a mutant strain, K_3-15,of Flavobacterium sp. 238-7 were investigated. A detergent-supplemented culture medium conststed of 60g of glycerol, 23g, of peptone, 3g of yeast extract, 7g of K_2HPO_4,5g of NaCl, 0.8g of MgSO_4・7H_2O, and 0.5g of Rikanon UA 5012 in 1l of tap water, pH7.0,was constructed. Amounts of MK-4,MK-6,and total MK in 1l of the medium were 101mg, 39mg, and 140mg, respectively, arter 7d of cultivation at 28℃. Further studies with some additives showed that the addition of cedar wood oil increased the productivity of MK, especially MK-4. In the presence of 0.1% Rikanon UA 5012 and 0.1% cedar wood oil, mutant strain K_3-15 produced 155mg./l (50mg/l intracellularly and 105mg/l extracellularly) of MK-4 and 27mg/l (16mg/l intracellularly and 11mg/l extracellularly) of MK-6,and the total amount of MK reache 182mg/l.

• 公益社団法人日本生物工学会の論文
• 1989-02-25

著者

• TAGUCHI Hisataka

  Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• Tani Yoshiki

  Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University

• Taguchi Hisataka

  Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University

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