α-Tocopherol Production by an Analog-resistant Strain of Euglena gracilis Z(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Improvement of the α-tocopherol productivity of Euglena gracilis Z was achieved by derivation of an analog-resistant strain. Among various compounds tested, β-2-thienylalanine was selected as the best inhibitor for derivation of high α-tocopherol-producing strains. A number of β-2-thienylalanine-resistant strains were obtained on UV irradiation with or without streptomycin treatment, and also on spontaneous mutation. One strain, S-T1, produced 4 times as much α-tocopherol as the wild type strain. As the result of optimization of the culture conditions, strain S-T1 produced 48.4 mg per 1 of culture broth or 1.8mg per gram of dry cell weight (DCW) of α-tocopherol intracellularly. The addition of homogentisate and ethanol increased the production to 130.3mg per 1 or 4.8mg per gram of DCW. Finally, a high amount of α-tocopherol, 180.4mg per 1 of 6.3mg per gram of DCW, was obtained with the feeding of glucose during the cultivation.
- 社団法人日本農芸化学会の論文
- 1989-09-23
著者
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TANI Yoshiki
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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OSUKA Shinoi
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Osuka Shinoi
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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