Isolation and Characterization of a Mutant of a Methanol Yeast, Candida boidinii S2, with Higher Formaldehyde Productivity
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概要
- 論文の詳細を見る
We isolated a mutant strain of a methanol-utilizing yeast, Candida boidinii S2, which shows improved formaldehyde productivity. The procedure for mutant screening consisted of; 1) induction of alcohol oxidase on a methanol-plate, 2) catabolite inactivation of alcohol oxidase on a glucose-plate, and 3) visualization of alcohol oxidase activity in a colony. One of the mutants, strain AOU-1, showed 1.7 times higher formaldehyde productivity and a higher growth rate on methanol than the parent strain. The high formaldehyde productivity was proved to be due to the high alcohol oxidase activity. No qualitative change of the enzyme was detected between the parent strain and mutant strain AOU-1. The high activity of mutant strain AOU-1 could be attributed to a quantitative change and a change in the rate of enzyme synthesis. Catabolite repression and inactivation of alcohol oxidase in the mutant were also discussed.
- 社団法人 日本農芸化学会の論文
著者
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Sakai Yasuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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TANI Yoshiki
Research Center for Cell and Tissue Culture, Kyoto University
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