Isolation of L-Methionine-Enriched Mutant of a Methylotrophic Yeast, Candida boidinii No.2201
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概要
- 論文の詳細を見る
Six strains of methylotrophic yeast were examined for production of L-methionine-enriched cells. Candida boidinii (kloeckera sp.) No. 2201,which accumulated 0.54 mg/g-dry cell weight (DCW) of free L-methionine (pool methionine), was selected as the parental strain for breeding L-methionine-rich mutants. Ethionine-resistant mutants were derived from the strain by UV irradiation. A mutant strain, E500-78,which was resistant to 500 μg/ml of DL-ethionine, accumulated 6.02 mg/g-DCW of pool methionine. The culture conditions for mutant strain E500-78 to increase pool methionine accumulation were optimized. As a result, the mutant strain accumulated 8.80 mg/g-DCW of pool methionine and contained 16.02 mg/g-DCW total methionine.
- 公益社団法人日本生物工学会の論文
- 1988-04-25
著者
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Kim Wang-jin
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University:department Of Fo
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Yang H‐c
Korea Univ. Seoul Kor
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Yang Han-chul
Department Of Biotechnology Faculty Of Engineering Tottori University
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Tani Yoshiki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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