ATP Production by Protoplasts of a Methanol Yeast, Candida boidinii (Kloeckera sp.) No. 2201
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概要
- 論文の詳細を見る
A phosphorylation system for formation of ATP from AMPby Zymolyase-treated cells of Candida boidinii (Kloeckera sp.) No. 2201 was developed as an ATP production process. This system was shown to be an energy conversion system, from a reduced C1-compoundto ATP through reduction of NAD+ and oxidative phosphorylation but not substrate level phosphorylation, together with phosphorylation of AMPto ADP. Reaction conditions for the ATP production were optimized in respect of substrate and coenzymeconcentrations, pHand temperature, osmotic pressure, and oxygen supply. Underthe optimal conditions, 26mM(13 g/liter) and 8.5 dim (4g/liter) of ATP were produced with methanol and formate as C1-substrate, respectively.
- 社団法人 日本農芸化学会の論文
著者
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Culture
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Mitani Yukio
Department Of Agricultural Chemistry Kyoto University
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